PMID: 6172387Aug 15, 1981Paper

Activation of the Epstein-Barr virus genome by 5-aza-cytidine in latently infected human lymphoid lines

International Journal of Cancer. Journal International Du Cancer
S A Ben-Sasson, G Klein

Abstract

Recent studies indicate that gene expression in higher eukaryotes is accompanied by a decrease in the frequency of 5-methyl cytosine residues around the activated site (Razin and Riggs, 1980). 5-aza-cytidine (5-aza-C) is an analogue that reduces cytidine methylation in DNA (Jones and Taylor, 1980) and has been reported to change the differentiation pattern of cultured mouse embryo cells (Taylor and Jones, 1979). We have tested its ability to activate the Epstein-Barr virus cycle in latently EBV-infected human lymphoid lines. After an incubation period of 6 to 8 h with the drug, early antigens (EA) were induced in a substantial fraction of the cells in all six lines tested that had a low rate of spontaneous viral antigen production. Optimal conditions for EA induction were defined. The efficiency of 5-aza-C was comparable to the inducing effect of iododeoxyuridine. EBV-DNA and EBNA positive virus-non-producer lines did not respond to 5-aza-C treatment. The findings are discussed in relation to the possibility that changes in EBV-gene expression may be related to the state of DNA methylation.

References

Jun 15, 1978·International Journal of Cancer. Journal International Du Cancer·R BerJ Zeuthen
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R C DesrosiersB Fleckenstein
Jul 15, 1972·International Journal of Cancer. Journal International Du Cancer·G KleinB Gothoskar
May 1, 1973·International Journal of Cancer. Journal International Du Cancer·B M Reedman, G Klein
Jan 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·Y Hinuma, J T Grace
Nov 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·P Gerber, S Lucas
Mar 15, 1973·International Journal of Cancer. Journal International Du Cancer·G Klein, L Dombos
Feb 1, 1973·Journal of the National Cancer Institute·C B Lozzio, B B Lozzio
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Sutter, W Doerfler
Nov 7, 1980·Science·A Razin, A D Riggs
Feb 1, 1964·Lancet·J V PULVERTAFT

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Citations

Jan 1, 1983·Journal of Cancer Research and Clinical Oncology·J Zeuthen
Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Oct 13, 2001·Trends in Microbiology·H Laman, C Boshoff
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M SzyfA Razin
Feb 1, 1983·The Journal of Cell Biology·J C Gasson, S Bourgeois
Jun 16, 2001·Antimicrobial Agents and Chemotherapy·S M MooreR F Ambinder
May 7, 2002·Journal of Virology·Lyndle GradovilleGeorge Miller
Nov 16, 2004·Journal of Virology·Yao ChangChing-Hwa Tsai
Jan 30, 2004·Journal of Virology·Wen-hai FengShannon C Kenney
Oct 1, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jianqing LinMichael A Carducci
Mar 1, 1989·Environmental Health Perspectives·L A Michalowsky, P A Jones
Oct 1, 2005·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Mee Soo Chang, Woo Ho Kim
Sep 5, 2014·The Journal of Pathology·Chrysoula DaskalogianniRobin Fåhraeus
Oct 13, 2006·Medical Hypotheses·Farhad F Shadan
Apr 14, 2012·Advances in Virology·Sajal K GhoshDouglas V Faller
Jun 20, 2012·Medical Hypotheses·Abraham R Liboff
Apr 11, 2007·Advances in Cancer Research·George MillerLyn Gradoville
Jul 1, 1988·Annales De L'Institut Pasteur. Virology·J BouchardM C Walker
Nov 1, 1983·The Journal of Experimental Zoology·P A JonesV Wilson
Mar 15, 1983·International Journal of Cancer. Journal International Du Cancer·G Bauer
Nov 17, 2004·Reviews in Medical Virology·Wolfgang Amon, Paul J Farrell
Dec 29, 2006·Chemistry & Biodiversity·Irene M Lagoja

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