Activation of the ferritin H enhancer, FER-1, by the cooperative action of members of the AP1 and Sp1 transcription factor families.

The Journal of Biological Chemistry
Y TsujiF M Torti

Abstract

We have previously reported that the adenovirus E1A oncogene represses the transcription of the H subunit of the mouse ferritin gene. Subsequent analyses defined FER-1, a 37-nucleotide sequence located 4.1 kilobases proximal to the start site of transcription, as the target of E1A-mediated transcriptional repression and as an enhancer of the ferritin H gene. FER-1 is composed of an AP1-like sequence followed by an element with dyad symmetry. To achieve maximal enhancer activity and transcriptional repression by E1A, both elements were essential. Using gel retardation assays, we now demonstrate that the binding complex for the AP1-like sequence of FER-1 contains JunD, FosB, and ATF1. Furthermore, JunD and FosB were able to activate FER-1 enhancer activity by transient cotransfection with ferritin H-chloramphenicol acetyltransferase reporter constructs. This augmented enhancer activity was inhibited by E1A. In addition, we have defined the minimal sequence in the dyad element of FER-1 required for protein interaction. This was determined to be a C-rich sequence to which Sp1 and Sp3 bind. Experiments with recombinant proteins indicate that members of both transcription factor families simultaneously bind FER-1. Taken together, the...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M Berg
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Hai, T Curran
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L L MillerF M Torti
May 31, 1990·Biochemical and Biophysical Research Communications·Y WeiF M Torti
Apr 11, 1988·Nucleic Acids Research·J L ImlerB Wasylyk
Jul 14, 1995·The Journal of Biological Chemistry·M Karin
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·A J UdvadiaJ M Horowitz
Jun 23, 1995·The Journal of Biological Chemistry·E L KwakF M Torti
Jul 1, 1993·Molecular and Cellular Biology·Y NakabeppuM Sekiguchi
Jan 12, 1996·The Journal of Biological Chemistry·G J MoffatC R Wolf
May 29, 2013·Molecular Systems Biology·Mathieu CourcellesPierre Thibault

❮ Previous
Next ❯

Citations

Jun 7, 2003·Advances in Enzyme Regulation·John WilkinsonFrank M Torti
Jan 1, 2003·Cancer Detection and Prevention·Regina Maki SasaharaMari Cleide Sogayar
Jun 15, 2007·The Journal of Biological Chemistry·Kenta IwasakiYoshiaki Tsuji
Mar 11, 2008·Antioxidants & Redox Signaling·Elizabeth L MacKenzieYoshiaki Tsuji
Feb 18, 2016·Experimental Eye Research·James K KubilusThomas F Linsenmayer
Nov 7, 2003·Developmental Cell·Meng C WangHeinrich Jasper
May 6, 2003·The Journal of Nutrition·Elizabeth C Theil
Oct 3, 2018·International Journal of Molecular Sciences·Ilenia AversaFrancesco Costanzo
Mar 8, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xinchao WangAndrew J Ghio
May 24, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrew J GhioLaura M Garrick
Dec 7, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anna L LaganGregory J Quinlan
Feb 1, 2000·The Journal of Biological Chemistry·C FerreiraC Beaumont
Nov 19, 2002·The Journal of Biological Chemistry·E Christine PietschSuzy V Torti
Mar 29, 2006·The Biochemical Journal·Abas H LaftahChris Tselepis

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.