Activation of the Gal1 gene of yeast by pairs of 'non-classical' activators

Current Biology : CB
Jason X ChengM Ptashne

Abstract

Eukaryotic transcriptional activators work by recruiting to DNA the transcriptional machinery, including protein complexes required for chromatin modification, transcription initiation, and elongation. Which of these complexes must be directly recruited to trigger transcription? We test various "non-classical" transcription activators (comprising a component of the transcriptional machinery fused to a DNA binding domain) for their abilities to activate transcription of a chromosomally integrated reporter in yeast. Among these newly constructed fusion proteins, none efficiently activated transcription when working on its own. However, in several instances transcription was activated by a pair of such fusion proteins tethered to adjacent sites on DNA. In each of these cases, one fusion protein bore a component of the SAGA complex, and the other bore a component of the Mediator complex. Transcription was also activated by certain tripartite fusion proteins comprising a Mediator and a SAGA component fused to a DNA binding domain. The results are consistent with the finding that the classical activator Gal4, working at the GAL1 promoter, activates transcription by (at least in part) independently recruiting SAGA and Mediator.

References

Apr 10, 1997·Nature·M Ptashne, A Gann
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·E Gonzalez-CoutoM Strubin
Jul 14, 1998·Molecular Cell·S S KohR A Young
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·L GaudreauM Ptashne
Jun 6, 2000·Trends in Biochemical Sciences·S Malik, R G Roeder
Nov 14, 2000·Molecular and Cellular Biology·J M ParkY J Kim
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·M R DotsonF J Asturias
Aug 4, 2001·Genes & Development·S R Bhaumik, M R Green
May 3, 2002·The Journal of Biological Chemistry·Michael J CarrozzaJerry L Workman
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhen LuMark Ptashne
Dec 7, 2002·The Journal of Biological Chemistry·Darius BalciunasHans Ronne
May 29, 2003·Molecular Cell·Gene O Bryant, Mark Ptashne
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Laurent KurasRoger D Kornberg
Feb 12, 2004·Genes & Development·Sukesh R BhaumikMichael R Green
Mar 10, 2004·Cell·Andrew D BasehoarB Franklin Pugh
Apr 29, 2004·Nature Structural & Molecular Biology·Steven Hahn

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