Activation of the human estrogen receptor by estrogenic and antiestrogenic compounds in Saccharomyces cerevisiae: a positive selection system

Gene
S P ShiauC L Hershberger

Abstract

The yeast URA3 gene was used as a reporter to investigate the activities of estrogenic and antiestrogenic compounds in yeast Saccharomyces cerevisiae. The control sequences of the wild type (wt) URA3 promoter were replaced with zero, two, or six copies of estrogen-response elements (ERE). Insertion of two and six copies of ERE rendered the expression of the URA3 gene to be dependent on the presence of the human estrogen receptor (ER) and the hormone 17beta-estradiol (E2). Two versions of the ER genes were constructed: a full-length wild-type ER (ERa-f) and a truncated ER with domains C, D, and E (ERcde). Both forms of the ER were able to activate the ERE-URA3 reporter in a hormone-dependent manner. The growth of yeast transformants were hormone-dependent when the reporter constructs were inserted into chromosomes using yeast integrating vectors (YIp) but not with the 2mu-based episomal (high-copy number, YEp) or centromeric (low-copy number, YCp) vectors. The integrated transformants were employed to investigate the effects of estrogenic and antiestrogenic compounds. The estrogenic compounds, E2, diethylstilbestrol (DES), and estrone (EST), activated expression of the reporter genes at 1 nM concentration, which is the same conc...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Dec 24, 1987·Cell·V KumarP Chambon
Jan 1, 1995·Annual Review of Medicine·R C RibeiroJ D Baxter
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·V CavaillèsM G Parker
Jan 1, 1994·Annual Review of Biochemistry·M J Tsai, B W O'Malley
Dec 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·B S KatzenellenbogenC K Wrenn
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yong ZhuPeter Thomas

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Citations

Jul 12, 2002·The Science of the Total Environment·Juliette LeglerAlbertinka J Murk
Apr 2, 1999·Nucleic Acids Research·P Balmelli-GallacchiD Picard
May 20, 2008·Expert Opinion on Drug Metabolism & Toxicology·José M Navas, Helmut Segner
May 25, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guido HasenbrinkHella Lichtenberg-Fraté
Mar 9, 2006·Best Practice & Research. Clinical Endocrinology & Metabolism·Ana M SotoCarlos Sonnenschein

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