Activation of the IGF-II gene by HBV-X protein requires PKC and p44/p42 map kinase signalings

Biochemical and Biophysical Research Communications
S Kang-ParkY I Lee

Abstract

We have recently shown that HBx protein, one of the causative agents of hepatocellular carcinomas, regulates Sp1 mediated transcription of insulin-like growth factor II promoter 4 (Lee et al. (1998) Oncogene 16, 2367-2380). Here we show that PKC and p44/p42MAPK signalings are required for the HBx-induced Sp1-mediated IGF-II P4 transcriptional activity since (i) PKC activation by PMA or PKC expression vector increases Sp1 phosphorylation and P4 activity in HBx-transfected HepG2 cells; (ii) PKC inhibition by PKC inhibitor Gö6976 reduces Sp1 phosphorylation, P4 activity, and IGF-II mRNA in HBx-transfected HepG2 cells; and (iii) the inhibition of MEK activation by U0126 reduces Sp1 phosphorylation, P4 activity and IGF-II mRNA in HBx-transfected HepG2 cells. These results demonstrate that PKC and p44/p42 MAPK cascades are the essential signaling pathways in Sp1-mediated IGF-II gene activation by HBx.

References

Jan 1, 1987·Annual Review of Biochemistry·D Ganem, H E Varmus
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Benn, R J Schneider
Jun 1, 1996·The Journal of Endocrinology·J A Toretsky, L J Helman
Oct 29, 1997·Molecular and Cellular Biology·N P Klein, R J Schneider
Mar 6, 1998·Molecular and Cellular Biology·I HavivY Shaul
Sep 17, 1998·The Journal of Biological Chemistry·Y H Lee, Y Yun
Oct 2, 1998·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·M A Buendia
Nov 21, 1998·The EMBO Journal·J Jiricny
Apr 30, 1999·Journal of Gastroenterology and Hepatology·I O NgM Ng
Jul 30, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alison H M ReidJohann S De Bono

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Citations

Apr 16, 2004·Virus Research·Jing-Chyi WangGuang-Yuh Hwang
Feb 29, 2008·Gene·Margaret C L TseChristopher H K Cheng
Dec 31, 2002·Biochemical and Biophysical Research Communications·Motoyuki OtsukaNaohiko Seki
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ayesha KhalidSaba Khaliq
Jan 1, 2012·Cells·Hildegard I D Mack, Karl Munger
Nov 20, 2003·Mass Spectrometry Reviews·Thomas Werner
Dec 12, 2003·World Journal of Gastroenterology : WJG·Xiao-Zhong WangQi-Ming Tao
Jan 14, 2011·Journal of Gastroenterology and Hepatology·Michael C Kew
Jun 30, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lydia BarréMohamed Ouzzine
Aug 13, 2002·The Journal of Biological Chemistry·Jinhua LiLi-Na Wei

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