Activation of the lutropin/choriogonadotropin receptor in MA-10 cells stimulates tyrosine kinase cascades that activate ras and the extracellular signal regulated kinases (ERK1/2).

Endocrinology
Koji Shiraishi, Mario Ascoli

Abstract

We show that activation of the recombinant lutropin/choriogonadotropin receptor (LHR) in mouse Leydig tumor cells (MA-10 cells) leads to the tyrosine phosphorylation of Shc (Src homology and collagen homology) and the formation of complexes containing Shc and Sos (Son of sevenless), a guanine nucleotide exchange factor for Ras. Because a dominant-negative mutant of Shc inhibits the LHR-mediated activation of Ras and the phosphorylation of ERK1/2, we conclude that the LHR-mediated phosphorylation of ERK1/2 is mediated, at least partially, by the classical pathway used by growth factor receptors. We also show that the endogenous epidermal growth factor receptor (EGFR) present in MA-10 cells is phosphorylated upon activation of the LHR. The LHR-mediated phosphorylation of the EGFR and Shc, the activation of Ras, and the phosphorylation of ERK1/2 are inhibited by expression of a dominant-negative mutant of Fyn, a member of the Src family kinases (SFKs) expressed in MA-10 cells and by PP2, a pharmacological inhibitor of the SFKs. These are also inhibited, but to a lesser extent, by AG1478, an inhibitor of the EGFR kinase. We conclude that the SFKs are responsible for the LHR-mediated phosphorylation of the EGFR and Shc, the formatio...Continue Reading

References

Jan 1, 1989·Annals of the New York Academy of Sciences·M Ascoli, D L Segaloff
May 13, 1998·Pharmacology & Therapeutics·D S Lawrence, J Niu
Dec 5, 1998·The International Journal of Biochemistry & Cell Biology·M D Resh
Apr 21, 1999·Current Opinion in Cell Biology·P O HackelA Ullrich
Sep 1, 1999·Chemistry & Biology·Y LiuK M Shokat
Dec 15, 2000·Oncogene·J D BjorgeD J Fujita
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y Yarden, M X Sliwkowski
Mar 30, 2001·The Journal of Biological Chemistry·R SegerA Amsterdam
Nov 17, 2001·The Journal of Biological Chemistry·John T SeykoraPaul L Stein
Apr 12, 2002·Endocrine Reviews·Mario AscoliDeborah L Segaloff
May 4, 2002·Nature Reviews. Molecular Cell Biology·Peter J Cullen, Peter J Lockyer
May 8, 2002·Nature Reviews. Molecular Cell Biology·Michael B Yaffe
Jun 21, 2002·Trends in Cell Biology·Philip J S Stork, John M Schmitt
Jan 2, 2003·Nature Reviews. Cancer·Julian Downward
Mar 5, 2003·Immunological Reviews·Li ZhangKodi S Ravichandran
Mar 18, 2003·Journal of Cell Science·J Thomas Parsons
May 3, 2003·Nature Reviews. Molecular Cell Biology·John F Hancock
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Thomas Holbro, Nancy E Hynes
Feb 3, 2004·Methods in Molecular Biology·Miranda van Triest, Johannes L Bos
Aug 24, 2004·Current Topics in Medicinal Chemistry·Stephen BrownShahriar Mobashery
Oct 19, 2004·Oncogene·Paul A BromannSara A Courtneidge
Oct 19, 2004·Oncogene·Deirdre K Luttrell, Louis M Luttrell
Nov 3, 2004·Trends in Cell Biology·Gretchen A RepaskyChanning J Der
Dec 8, 2004·The Journal of Biological Chemistry·Weijie LiJohn Seykora
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Satyajit K MitraDavid D Schlaepfer
Apr 20, 2005·Molecular and Cellular Endocrinology·Ilpo HuhtaniemiMatti Poutanen
Apr 23, 2005·Biochemical and Biophysical Research Communications·Robert Roskoski
Aug 2, 2005·Trends in Endocrinology and Metabolism : TEM·Minnie Hsieh, Marco Conti
Aug 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Axel P N Themmen
Aug 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Susana B Rulli, Ilpo Huhtaniemi
Aug 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·T Rajendra Kumar
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Michelle JamnongjitStephen R Hammes

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Citations

Nov 3, 2009·Molecular Endocrinology·Heng-Yu Fan, Joanne S Richards
Nov 22, 2011·Molecular and Cellular Endocrinology·Marco ContiJeong Su Oh
Apr 23, 2013·Chemical Biology & Drug Design·Rajshri M NavalakheSmita D Mahale
Oct 23, 2016·General and Comparative Endocrinology·Dilip MukherjeeDhynendra Kumar
Jan 20, 2017·Endocrine Journal·Koji Shiraishi, Hideyasu Matsuyama

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