Activation of the mismatch-specific endonuclease EndoMS/NucS by the replication clamp is required for high fidelity DNA replication

Nucleic Acids Research
Sonoko IshinoHannu Myllykallio

Abstract

The mismatch repair (MMR) system, exemplified by the MutS/MutL proteins, is widespread in Bacteria and Eukarya. However, molecular mechanisms how numerous archaea and bacteria lacking the mutS/mutL genes maintain high replication fidelity and genome stability have remained elusive. EndoMS is a recently discovered hyperthermophilic mismatch-specific endonuclease encoded by nucS in Thermococcales. We deleted the nucS from the actinobacterium Corynebacterium glutamicum and demonstrated a drastic increase of spontaneous transition mutations in the nucS deletion strain. The observed spectra of these mutations were consistent with the enzymatic properties of EndoMS in vitro. The robust mismatch-specific endonuclease activity was detected with the purified C. glutamicum EndoMS protein but only in the presence of the β-clamp (DnaN). Our biochemical and genetic data suggest that the frequently occurring G/T mismatch is efficiently repaired by the bacterial EndoMS-β-clamp complex formed via a carboxy-terminal sequence motif of EndoMS proteins. Our study thus has great implications for understanding how the activity of the novel MMR system is coordinated with the replisome and provides new mechanistic insight into genetic diversity and mu...Continue Reading

References

Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R M Schaaper, R L Dunn
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·B P DalrympleP A Jennings
May 14, 2003·Applied Microbiology and Biotechnology·M Ikeda, S Nakagawa
Dec 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Damien PortevinChristophe Guilhot
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Aug 28, 2007·Journal of Molecular Biology·Laurence Meslet-CladiéreHannu Myllykallio
Jul 18, 2009·The EMBO Journal·Bin RenHannu Myllykallio
Sep 29, 2009·The Journal of Biological Chemistry·Anna PluciennikPaul Modrich
Oct 23, 2009·Nature·Stephen P Jackson, Jiri Bartek
Aug 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anna PluciennikPaul Modrich
Mar 21, 2012·The Journal of Biological Chemistry·Christophe CrezeDidier Flament
Apr 3, 2013·Cold Spring Harbor Perspectives in Biology·Josef Jiricny
Oct 6, 2015·Annual Review of Genetics·Thomas A Kunkel, Dorothy A Erie
Jan 8, 2016·DNA Repair·Khek-Chian ThamJoyce H G Lebbink
Feb 13, 2016·PLoS Genetics·Matthew J BlowRichard J Roberts
Mar 24, 2016·Nucleic Acids Research·Sonoko IshinoYoshizumi Ishino
Apr 28, 2016·World Journal of Microbiology & Biotechnology·Volker F WendischElvira Sgobba
Jun 4, 2016·Molecular Cell·Rais A Ganai, Erik Johansson
Nov 3, 2016·Structure·Setsu NakaeTsuyoshi Shirai
Nov 3, 2016·Structure·Mariko Ariyoshi, Kosuke Morikawa
Jan 28, 2017·Nature Communications·A Castañeda-GarcíaJ Blázquez
Feb 9, 2018·Nature·Isaac J KimseyHashim M Al-Hashimi

❮ Previous
Next ❯

Citations

Feb 13, 2019·Molecular Microbiology·Claire BertrandPierre Leblond
Jun 3, 2020·Journal of Bacteriology·Rupal JainDennis W Grogan
Oct 20, 2019·International Journal of Molecular Sciences·Yanjun Zhang, Shengyou Huang
Jul 28, 2020·Frontiers in Microbiology·Likui ZhangPhilippe M Oger
Dec 16, 2020·FEMS Microbiology Reviews·Rhastin A D CastroSebastien Gagneux
Oct 30, 2020·Biomolecules·Craig J Marshall, Thomas J Santangelo
Jun 3, 2021·Cells·Esmeralda Cebrián-SastreAlfredo Castañeda-García
Oct 21, 2021·Biotechnology Letters·Fa-Yu YangFeng Gu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Seikagaku. The Journal of Japanese Biochemical Society
Motoshi Suzuki, Shunji Izuta
Current Opinion in Microbiology
Zvi Kelman, Malcolm F White
Advances in Experimental Medicine and Biology
A R Fersht
Annual Review of Biochemistry
H Echols, M F Goodman
© 2022 Meta ULC. All rights reserved