Activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway by conventional, novel, and atypical protein kinase C isotypes.

Molecular and Cellular Biology
D C SchönwasserP J Parker

Abstract

Phorbol ester treatment of quiescent Swiss 3T3 cells leads to cell proliferation, a response thought to be mediated by protein kinase C (PKC), the major cellular receptor for this class of agents. We demonstrate here that this proliferation is dependent on the activation of the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) cascade. It is shown that dominant-negative PKC-alpha inhibits stimulation of the ERK/MAPK pathway by phorbol esters in Cos-7 cells, demonstrating a role for PKC in this activation. To assess the potential specificity of PKC isotypes mediating this process, constitutively active mutants of six PKC isotypes (alpha, beta, delta, epsilon, eta, and zeta) were employed. Transient transfection of these PKC mutants into Cos-7 cells showed that members of all three groups of PKC (conventional, novel, and atypical) are able to activate p42 MAPK as well as its immediate upstream activator, the MAPK/ERK kinase MEK-1. At the level of Raf, the kinase that phosphorylates MEK-1, the activation cascade diverges; while conventional and novel PKCs (isotypes alpha and eta) are potent activators of c-Raf1, atypical PKC-zeta cannot increase c-Raf1 activity, stimulating MEK by an independent mec...Continue Reading

References

Sep 23, 1991·FEBS Letters·P D Adams, P J Parker
Jan 1, 1991·Pharmacology & Therapeutics·S Stabel, P J Parker
Nov 26, 1990·European Journal of Biochemistry·C J PearsP J Parker
Dec 15, 1990·The Biochemical Journal·S AhmedL Lim
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A J RossomandoT W Sturgill
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D K MorrisonT M Roberts
Jun 15, 1987·The Biochemical Journal·S YoungS Stabel
Dec 1, 1985·Molecular and Cellular Biology·G I EvanJ M Bishop
Apr 28, 1995·The Journal of Biological Chemistry·E M GenotD A Cantrell
Jan 1, 1995·Trends in Biochemical Sciences·B M Burgering, J L Bos
Jul 1, 1994·Trends in Biochemical Sciences·J AvruchJ M Kyriakis
Feb 24, 1994·Nature·F ZhangE J Goldsmith
Feb 1, 1994·Trends in Biochemical Sciences·L V Dekker, P J Parker
Apr 15, 1993·The Biochemical Journal·H Hug, T F Sarre
Sep 12, 1996·Nature·C J Marshall

❮ Previous
Next ❯

Citations

Aug 3, 2011·The Journal of Cell Biology·Patricia L Martínez-MoralesAixa V Morales
Mar 1, 2000·British Journal of Pharmacology·G LenzJ T Neary
Nov 17, 2004·Molecular Carcinogenesis·Alejandro J UrtregerElisa D Bal de Kier Joffé
May 9, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Vivian SoetiknoKenichi Watanabe
Mar 24, 2011·Experimental Eye Research·Jerry G WebbCraig E Crosson
Oct 19, 2010·Bone·Akio NakuraHideki Yoshikawa
May 4, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Elina EkokoskiRaimo K Tuominen
Nov 26, 2009·Biochimica Et Biophysica Acta·James W SimpkinsJames A Dykens
May 5, 2009·Frontiers in Neuroendocrinology·James W SimpkinsShao-Hua Yang
Oct 28, 2008·Experimental Cell Research·Meredith V BrownAlbert B Reynolds
Sep 10, 2008·Experimental Cell Research·Sarah Morgan, Jennifer R Grandis
Nov 23, 2007·European Journal of Pharmacology·David NilssonMalin Malmsjö
Jun 29, 2007·The International Journal of Biochemistry & Cell Biology·Roberto BiancoGiampaolo Tortora
Jul 9, 2004·British Journal of Pharmacology·Marie HenrikssonLars Edvinsson
Jun 25, 2009·Journal of Cellular and Molecular Medicine·Roberto RoncaPatrizia Dell'Era
Dec 5, 2008·Journal of Neurochemistry·Kun Don YiJames W Simpkins
May 15, 2012·Pigment Cell & Melanoma Research·Mitchell F Denning
Feb 16, 2011·The Journal of Physiology·Michael B Reid, Jennifer S Moylan
May 12, 2010·Journal of Cellular Physiology·Alexandra A AndersonDavid J Mann
Apr 17, 2013·The FEBS Journal·Carsten Schmitz-Peiffer
Jan 4, 2012·Molecular Carcinogenesis·Claudio LuparelloAlessandra Longo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.