Activation of the mouse cytokeratin A (endo A) gene in teratocarcinoma F9 cells by the histone deacetylase inhibitor Trichostatin A

FEBS Letters
T MiyashitaA Matsushiro

Abstract

Treatment of cultured cells with sodium butyrate, that is the histone deacetylase inhibitor, induces the histone hyperacetylation and the expressions of various mammalian genes without affecting the level of protein synthesis. However, butyrate is a non-specific inhibitor of deacetylase because of its effects on various other enzymes and nuclear proteins other than histones. On the other hand, Trichostatin A (TSA) was recently found to be a potent and specific inhibitor of histone deacetylase. We examined the effect of TSA on the expression of mouse cytokeratin A (endo A). TSA increased endoA expression in F9 cells, and was effective at a much lower concentration than sodium butyrate. We also examined the changes of chromatin structure induced by the two drugs by a DNase I-hypersensitivity assay. Both drugs induced the formation of a DNase I-hypersensitive site (DH site) in only the promoter region. The precise mechanism(s) by which the two drugs increase endoA gene expression is unknown, but these results suggest that endoA expression is induced by inhibition of histone deacetylase and that the effect is at the transcriptional level.

References

Sep 3, 1976·Science·H Weintraub, M Groudine
May 25, 1991·Nucleic Acids Research·Y TakemotoM Nozaki
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E H BresnickG L Hager
Feb 1, 1990·Trends in Genetics : TIG·J Svaren, R Chalkley
Mar 23, 1990·Cell·J Tazi, A Bird
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T A Chen, V G Allfrey
Oct 1, 1983·Developmental Biology·R G OshimaL H Shevinsky
Oct 8, 1993·Cell·B M Turner

❮ Previous
Next ❯

Citations

May 23, 1998·Annual Review of Pharmacology and Toxicology·T J Monks, S S Lau
Sep 4, 2004·Environmental Health Perspectives·Kim KultimaMichael Stigson
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R L GendronH Paradis
Mar 14, 1997·The Journal of Biological Chemistry·J K JeongT J Monks
Dec 31, 2003·The Journal of Biological Chemistry·Tzeng-Horng LeuMing-Chei Maa
Sep 1, 1997·Molecular and Cellular Biology·S BartlC Seiser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.