Activation of the mTOR signalling pathway is required for pancreatic growth in protease-inhibitor-fed mice.

The Journal of Physiology
Stephen J CrozierJohn A Williams

Abstract

Cholecystokinin (CCK)-induced pancreatic growth in mice involves parallel increases in DNA and protein. The mammalian target of rapamycin (mTOR) signalling pathway regulates mRNA translation and its activation is implicated in growth of various tissues. The aim of this study was to elucidate whether mTOR activation is required for pancreatic growth in a mouse model of increased endogenous CCK release. In mice fed chow containing the synthetic protease inhibitor camostat, protein synthetic rates and phosphorylation of two downstream targets of mTOR, eukaryotic initiation factor 4E binding protein 1 (4E-BP1) and the ribosomal protein S6 (S6), increased in comparison with fasted controls. The camostat-induced increases in protein synthesis and 4E-BP1 and S6 phosphorylation were almost totally abolished by administration of the mTOR inhibitor rapamycin 1 h prior to camostat feeding. In contrast, the phosphorylation of ERK1/2 and JNK and the expression of the early response genes c-jun, c-fos, ATF3 and egr-1 induced by camostat feeding were not affected by rapamycin. In mice fed camostat for 7 days, the ratio of pancreatic to body weight increased by 143%, but when rapamycin was administered daily this was reduced to a 22% increase....Continue Reading

Associated Clinical Trials

Feb 18, 2021·Shaffer Mok

References

Feb 1, 1991·Acta Physiologica Scandinavica·K LundholmL Lindström
Aug 1, 1968·European Journal of Pharmacology·E Meeter, O L Wolthuis
Jun 1, 1984·Environmental Health Perspectives·E E McGuinnessK G Wormsley
Jan 1, 1980·Endocrinology·A B Dembinski, L R Johnson
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·L M GravesJ C Lawrence
Jul 1, 1997·The American Journal of Physiology·M J BragadoJ A Williams
Aug 26, 1998·The Journal of Biological Chemistry·G E GroblewskiJ A Williams
Dec 2, 1999·Experimental Cell Research·A Dufner, G Thomas
Sep 23, 2000·American Journal of Physiology. Endocrinology and Metabolism·O J ShahL S Jefferson
Oct 13, 2000·European Journal of Biochemistry·O Meyuhas
Jan 24, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Maria Dolors SansJohn A Williams
Mar 8, 2003·International Journal of Gastrointestinal Cancer·Maria Dolors Sans, John A Williams
May 30, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Maria Dolors SansJohn A Williams
Jun 13, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Claus SchäferGuy E Groblewski
Dec 20, 2003·Biochemical and Biophysical Research Communications·Scot R Kimball, Leonard S Jefferson
Dec 20, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mitsuo TashiroJohn A Williams
Mar 27, 2004·American Journal of Physiology. Cell Physiology·Maria Dolors Sans, John A Williams
May 1, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Maria Dolors SansJohn A Williams
May 26, 2004·Biochemical and Biophysical Research Communications·Maria Dolors SansJohn A Williams
Dec 9, 2004·Current Opinion in Clinical Nutrition and Metabolic Care·Joseph AvruchSara Ortiz-Vega
Feb 4, 2005·Current Opinion in Gastroenterology·Yan Bi, John A Williams
Mar 23, 2005·Current Opinion in Cell Biology·Dietmar E Martin, Michael N Hall
Aug 5, 2005·American Journal of Physiology. Cell Physiology·Gustavo A NaderKaryn A Esser

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Citations

Nov 6, 2007·Molecular Biology of the Cell·Grzegorz T GurdaJohn A Williams
Mar 31, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lili GuoJohn A Williams
Jun 12, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Megan D BaumlerGuy E Groblewski
Aug 20, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Maria Dolors SansJohn A Williams
Aug 28, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Stephen J CrozierJohn A Williams
May 12, 2009·Gastroenterology·Stephen J CrozierJohn A Williams
Aug 23, 2018·American Journal of Physiology. Cell Physiology·Sidney Abou SawanDaniel R Moore
Jun 6, 2007·Current HIV/AIDS Reports·Anna K PersonNathan M Thielman
Dec 2, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Megan D BaumlerGuy E Groblewski
Nov 11, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lili GuoJohn A Williams
Mar 22, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Stephen J CrozierJohn A Williams
Aug 12, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Bryan J HoltzJohn A Williams

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