PMID: 2109635Feb 27, 1990Paper

Activation of the nickel-deficient carbon monoxide dehydrogenase from Rhodospirillum rubrum: kinetic characterization and reductant requirement

Biochemistry
S A EnsignP W Ludden

Abstract

The requirements for and kinetics of the activation of the nickel-deficient (apo) CO dehydrogenase from Rhodospirillum rubrum by exogenous nickel have been investigated. The activation is strictly dependent upon the presence of a low-potential one-electron reductant. Sodium dithionite and reduced methylviologen (E degrees' = -440 mV) are suitable reductants, whereas reduced indigo carmine (E degrees' = -125 mV) and the two-electron reductants sodium borohydride, NADH, and dithiothreitol are ineffective in stimulating activation. The midpoint potential for activation was observed at approximately -475 mV. The ability of a reductant to stimulate activation is correlated with the reduced state of the enzyme Fe4-S4 centers. The activation follows apparent first-order kinetics in a saturable fashion, yielding a rate constant of 0.157 min-1 at saturating concentration of nickel. The initial rate at which the enzyme is activated by NiCl2 is also a saturable process, yielding a dissociation constant (KD) of 755 microM for the initial association of nickel and enzyme. Cadmium(II), zinc(II), cobalt(II), and iron(II) are competitive inhibitors of nickel activation with inhibition constants of 2.44, 4.16, 175, and 349 microM, respectively....Continue Reading

References

Jan 1, 1979·Methods in Enzymology·W W Cleland
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D BonamP W Ludden
Aug 11, 1987·Biochemistry·C T Walsh, W H Orme-Johnson
Apr 1, 1964·Biochemistry·W W CLELAND
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P Saint-MartinJ J Moura

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Citations

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G O TanK O Hodgson
Aug 11, 2015·Biochimica Et Biophysica Acta·Jessica Hadj-SaïdSébastien Dementin
Nov 25, 2006·Biochimica Et Biophysica Acta·Steven W SingerPaul W Ludden
Jun 28, 2003·FEMS Microbiology Reviews·Scott B Mulrooney, Robert P Hausinger
Feb 23, 1996·The Journal of Biological Chemistry·R M AllenV K Shah
Jul 13, 2019·The Journal of Biological Chemistry·Elizabeth C WittenbornCatherine L Drennan
Jul 27, 1999·Journal of Bacteriology·R K Watt, P W Ludden
Aug 29, 2009·Chemical Reviews·Yanjie Li, Deborah B Zamble
Feb 12, 2004·Chemical Reviews·Jason Kuchar, Robert P Hausinger

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