Activation of the Nrf2-ARE Signaling Pathway Prevents Hyperphosphatemia-Induced Vascular Calcification by Inducing Autophagy in Renal Vascular Smooth Muscle Cells.

Journal of Cellular Biochemistry
Li YaoZi-Tong Sheng

Abstract

This study investigates the effect of nuclear factor erythroid 2-related factor 2-antioxidant response element (Nrf2-ARE) signaling pathway in vascular calcification (VC) via inducing Autophagy in renal vascular smooth muscle cells (VSMCs). VSMCs were assigned into six experimental groups: the normal control, high phosphorus, si-negative control (si-NC), Nrf2-siRNA, over-expressed Nrf2, and negative control (NC) groups. RT-PCR was applied to detect the mRNA expressions of the desired Nrf2-ARE signaling pathway-related genes (Nrf2, NQO-1, HO-1, γ-GCS). The protein products of these genes: apoptosis-related genes (LC3I and LC3II), osteogenic marker proetins (Runt-related transcription factor 2) Runx2 and BMP2 were all detected by Western blotting. Autophagosomes in VSMCs were observed under a transmission electron microscope. We discovered an increased calcium ion concentration and upregulated Runx2, BMP2, Nrf2, HO-1, γ-GCS, NQO-1, and LC3II/LC3I expressions in the high phosphorous, si-NC and Nrf2-siRNA, and NC groups, compared with the normal control group. Compared to the high phosphorus and si-NC groups, higher levels of Runx2 and BMP2 but decreased Nrf2, HO-1, γ-GCS, NQO-1, and LC3II/LC3I expressions were detected in the Nrf2...Continue Reading

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Citations

Jul 9, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun-Seop SongJoseph Loscalzo
Jul 16, 2019·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Peter StenvinkelPaul G Shiels
Dec 13, 2019·Oxidative Medicine and Cellular Longevity·Xueyan ZhangQingnan He
Jun 5, 2020·Vascular Health and Risk Management·Sepideh Zununi VahedMohammadreza Ardalan
Aug 25, 2020·Oxidative Medicine and Cellular Longevity·Liangkai ChengCaiyan Wang
May 16, 2020·Cardiology Research and Practice·Xueyan ZhangMingyi Zhao
Dec 2, 2020·International Journal of Molecular Sciences·Cédric H G NeutelPieter-Jan Guns
Jan 7, 2021·Toxins·Nikolaos C KyriakidisPeter Stenvinkel
Feb 26, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Samsul ArefinPeter Stenvinkel
Dec 18, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Donghua JinKang Xun

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