PMID: 11917081Mar 28, 2002Paper

Activation of the promoters of Arabidopsis genes for the branched-chain alpha-keto acid dehydrogenase complex in transgenic tobacco BY-2 cells under sugar starvation

Plant & Cell Physiology
Yuki FujikiA Watanabe

Abstract

Sugar starvation exerted by sub-10 mM levels of sucrose on Arabidopsis T87 suspension-cultured cells triggered marked accumulation of the transcripts of genes for E1beta and E2 subunit of the branched-chain alpha-keto acid dehydrogenase complex. Similar levels of sugar starvation increased the luciferase activity in transgenic tobacco BY-2 lines expressing the Arabidopsis E1beta- or E2-promoter-luciferase fusion gene. These results indicate that sugar levels tightly regulate the E1beta and E2 promoter activity in the heterologous plant system. We further showed in the transgenic tobacco BY-2 lines that sugar-starvation-induced activation of the E1beta and E2 promoters was prevented by K-252a, an inhibitor of Ser/Thr protein kinase, and was enhanced by okadaic acid, an inhibitor of protein phosphatases. By contrast, the cauliflower mosaic virus 35S promoter activity in sugar-starved BY-2 cells was not significantly affected by K-252a and only slightly enhanced by okadaic acid. Taken together, we propose that transcriptional activation of genes for the branched-chain alpha-keto acid dehydrogenase complex and its modulation by specific protein kinases/phosphatases are of critical importance in branched-chain amino acid catabolism ...Continue Reading

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Citations

Aug 26, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M MalatrasiN Marmiroli
Aug 18, 2004·Plant Physiology·Anthony L ContentoDiane C Bassham
Mar 5, 2013·Plant Physiology·Masanori IzumiHiroyuki Ishida
Aug 13, 2014·Journal of Experimental Botany·Réjane Pratelli, Guillaume Pilot
Jan 10, 2012·Journal of Plant Physiology·Andrej KochevenkoWagner L Araújo
Mar 30, 2011·Plant Physiology and Biochemistry : PPB·Hiroto HiranoMasami Sekine
Feb 9, 2010·Plant Physiology and Biochemistry : PPB·Anthony L Contento, Diane C Bassham
Jun 16, 2012·Plant, Cell & Environment·Bożena Szal, Anna Podgórska
May 9, 2014·Frontiers in Plant Science·Peter Schertl, Hans-Peter Braun
Jan 1, 2008·The Arabidopsis Book·A Harvey MillarJames Whelan

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