Activation of the prostaglandin D2 metabolic pathway in Crohn's disease: involvement of the enteric nervous system

BMC Gastroenterology
Anne-Gaelle Le LouppDamien Masson

Abstract

Recent works provide evidence of the importance of the prostaglandin D2 (PGD2) metabolic pathway in inflammatory bowel diseases. We investigated the expression of PGD2 metabolic pathway actors in Crohn's disease (CD) and the ability of the enteric nervous system (ENS) to produce PGD2 in inflammatory conditions. Expression of key actors involved in the PGD2 metabolic pathway and its receptors was analyzed using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in colonic mucosal biopsies of patients from three groups: controls, quiescent and active CD patients. To determine the ability of the ENS to secrete PGD2 in proinflammatory conditions, Lipocalin-type prostaglandin D synthase (L-PGDS) expression by neurons and glial cells was analyzed by immunostaining. PGD2 levels were determined in a medium of primary culture of ENS and neuro-glial coculture model treated by lipopolysaccharide (LPS). In patients with active CD, inflamed colonic mucosa showed significantly higher COX2 and L-PGDS mRNA expression, and significantly higher PGD2 levels than healthy colonic mucosa. On the contrary, peroxysome proliferator-activated receptor Gamma (PPARG) expression was reduced in inflamed colonic mucosa of CD patients with...Continue Reading

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Citations

Jul 19, 2016·Oral Diseases·S Sommakia, O J Baker
Jan 1, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Aaron K Chow, Brian D Gulbransen
Aug 5, 2017·Frontiers in Medicine·Miriam PeinhauptAkos Heinemann
Oct 25, 2020·Biological Trace Element Research·Mostafa Vaghari-TabariAmir Ghorbanihaghjo
May 11, 2021·Cellular and Molecular Gastroenterology and Hepatology·Camille PochardMalvyne Rolli-Derkinderen

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Methods Mentioned

BETA
biopsies
biopsy
PCR
dissection
IEC

Software Mentioned

RotorGene
Prism
GraphPad

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