Activation of the rat follicle-stimulating hormone receptor promoter by steroidogenic factor 1 is blocked by protein kinase a and requires upstream stimulatory factor binding to a proximal E box element

Molecular Endocrinology
L L Heckert

Abstract

The receptor for the pituitary glycoprotein hormone FSH (FSHR) and the nuclear hormone receptor steroidogenic factor 1 (SF-1) play important roles in control of the hypothalamic-pituitary- gonadal axis. FSHR is essential for integrating the pituitary FSH signal to gonadal response, while SF-1 is an important transcriptional regulator of many genes that function within this axis and is essential for the development of gonads and adrenal glands. Given the critical role of SF-1 in regulation of the gonads and the coexpression of FSHR and SF-1 in Sertoli and granulosa cells, we examined the ability of SF-1 to regulate transcription of the FSHR gene. We found that SF-1 stimulated rat FSHR promoter activity in a dose-dependent and promoter-specific manner. Examination of various promoter deletion mutants indicated that SF-1 acts through the proximal promoter region and upstream promoter sequences. An E box element within the proximal promoter is essential for activation of the FSHR promoter by SF-1. This element binds the transcriptional regulators USF1 and USF2 (upstream stimulatory factors 1 and 2) but not SF-1, as shown by electrophoretic mobility shift assays. In addition, functional studies identified a requirement for the USF p...Continue Reading

References

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Citations

Dec 16, 2003·Molecular and Cellular Biology·Ning Lei, Leslie L Heckert
Apr 9, 2005·Molecular Endocrinology·Brian P Hermann, Leslie L Heckert
Jan 1, 2008·Molecular Endocrinology·Hiroki UtsunomiyaSerdar E Bulun
May 23, 2002·Recent Progress in Hormone Research·Leslie L Heckert, Michael D Griswold
Sep 19, 2008·General and Comparative Endocrinology·Gersende Maugars, Monika Schmitz
Nov 18, 2008·Biochemical Pharmacology·Barbara Oesch-Bartlomowicz, Franz Oesch
Jan 21, 2015·Animal Reproduction Science·Wangjun WuHonglin Liu
Nov 14, 2006·Molecular and Cellular Endocrinology·Brian P HermannLeslie L Heckert
Nov 7, 2006·Molecular and Cellular Endocrinology·Brian P Hermann, Leslie L Heckert
Jan 30, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Simon M PlummerClifford R Elcombe
Aug 27, 2010·Biology of Reproduction·Jitu W GeorgeLeslie L Heckert
Dec 1, 2020·The FEBS Journal·Antara A BanerjeeSmita D Mahale
Aug 19, 2003·The Journal of Biological Chemistry·Clara I RodríguezManuel Fresno

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