Activation of the Salmonella typhimurium Mrr protein

Biochemical and Biophysical Research Communications
A AertsenC W Michiels

Abstract

The Mrr protein of Escherichia coli K12 is a cryptic type IV restriction endonuclease with specificity for methylated DNA. Recently it was discovered that endogenous activation of E. coli Mrr could be triggered by high pressure stress, resulting in the generation of double strand breaks in the host chromosome and concomitant induction of the SOS response. In this report we focused on Mrr activity of Salmonella Typhimurium LT2, and although we surprisingly found no evidence of high pressure induced activation, a large number of constitutively activated Mrr mutants could be isolated when the mrr gene was routinely cloned in an expression vector. Analysis of several spontaneous mutants revealed different single mutations that rendered the Mrr protein constitutively active. Moreover, a spontaneous S. Typhimurium mutant could be isolated that displayed an increased basal SOS induction because of a point mutation in the chromosomal mrr gene. Based on these findings the physiological role of Mrr in the cell is discussed.

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Citations

Jul 12, 2012·Biotechnology Letters·Junlin Yang, Yong Yang
Oct 2, 2009·Nucleic Acids Research·Rachel M SmithMark D Szczelkun
Apr 21, 2011·Nucleic Acids Research·Mehari Tesfazgi MebrhatuAbram Aertsen
Jan 26, 2010·Applied and Environmental Microbiology·Thorsten AllersMoshe Mevarech
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Kommireddy Vasu, Valakunja Nagaraja
Oct 28, 2008·Biochemical and Biophysical Research Communications·Jerzy OrlowskiAbram Aertsen
Jan 30, 2013·Critical Reviews in Microbiology·Mehari Tesfazgi MebrhatuAbram Aertsen
Jun 14, 2014·FEMS Microbiology Reviews·Zeynep Baharoglu, Didier Mazel
Mar 19, 2020·Micromachines·Phalguni Tewari KumarJeroen Lammertyn
Nov 22, 2020·DNA Repair·Anaïs C BourgesNathalie Declerck
Nov 1, 2012·EcoSal Plus·Ashley B Williams, Patricia L Foster

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