Activation of the transcription factor nuclear factor-kappa B in uterine luminal epithelial cells by interleukin 1 Beta 2: a novel interleukin 1 expressed by the elongating pig conceptus

Biology of Reproduction
Daniel MathewMatthew Lucy

Abstract

Conceptus mortality is greatest in mammals during the peri-implantation period, a time when conceptuses appose and attach to the uterine surface epithelium while releasing proinflammatory molecules. Interleukin 1 beta (IL1B), a master proinflammatory cytokine, is released by the primate, rodent, and pig blastocyst during the peri-implantation period and is believed to be essential for establishment of pregnancy. The gene encoding IL1B has duplicated in the pig, resulting in a novel gene. Preliminary observations indicate that the novel IL1B is specifically expressed by pig conceptuses during the peri-implantation period. To verify this, IL1B was cloned from mRNA isolated from Day 12 pig conceptuses and compared with IL1B cloned from mRNA isolated from pig peripheral blood leukocytes (PBLs). The pig conceptuses, but not the PBLs, expressed a novel IL1B, referred to here as interleukin 1 beta 2 (IL1B2). Porcine endometrium was treated with recombinant porcine interleukin 1 beta 1 (IL1B1), the prototypical cytokine, and IL1B2 proteins. Immunohistochemistry and real-time RT-PCR were used to measure activation of nuclear factor-kappa B (NFKB) and NFKB-regulated transcripts, respectively, within the endometrium. Both IL1B1 and IL1B2 ...Continue Reading

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Citations

Apr 11, 2017·Molecular Reproduction and Development·Rodney D GeisertThomas E Spencer
Apr 11, 2017·Molecular Reproduction and Development·Kevin D Wells, Randall S Prather
Feb 21, 2018·BJOG : an International Journal of Obstetrics and Gynaecology·A Sakowicz
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Oct 6, 2021·Biology of Reproduction·Destiny N JohnsRodney D Geisert

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Methods Mentioned

BETA
electrophoresis
PCR
blood collection
In Vitro
lavage
nuclear translocation

Software Mentioned

ImageJ
Statistical Analysis System ( SAS
blastn
blastp
Sequence Detection
SAS
Microsoft Office
BLAST

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