PMID: 8584445Jan 1, 1996Paper

Activation of the volume-sensitive chloride current in vascular endothelial cells requires a permissive intracellular Ca2+ concentration

Pflügers Archiv : European journal of physiology
G SzücsB Nilius

Abstract

Combined patch clamp and Ca2+-measurements (Fura-2) were used to study the dependence of volume-activated Cl--currents (ICl,vol) of endothelial cells from bovine pulmonary artery on the intracellular Ca2+-concentration [Ca2+]i. Loading the cells with high concentrations of EGTA or BAPTA via ruptured membrane patches or by preincubating them with 50 microM BAPTA-AM caused a substantial decrease of ICl,vol. This reduction was independent of the activation state of the current: the current amplitude was not only diminished if [Ca2+]i was lowered at the peak of the volume-activated current, but this low Ca2+-concentration also prevented activation of the current by a second hypotonic challenge.ICl,vol is already maximally activated at intracellular Ca2+-concentrations between 50 and 100 nmol/l, a further increase of [Ca2+]i does not affect the size of ICl,vol.These results indicate that a sustained full activation of ICl,vol in endothelial cells requires submicromolar concentrations of Ca2+, and that changes in [Ca2+]i do not modulate the current.

References

Mar 19, 1992·Nature·M PaulmichlD Clapham
May 1, 1995·The Journal of General Physiology·P S Jackson, K Strange
Apr 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M A ValverdeF V Sepúlveda
May 1, 1994·The Journal of General Physiology·B NiliusG Droogmans
Oct 1, 1995·Kidney International·K Strange, P S Jackson

❮ Previous
Next ❯

Citations

Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Mar 5, 2003·Molecular and Biochemical Parasitology·Peter RohloffR Docampo
Jan 4, 2001·Neurochemistry International·S Morales-MuliaH Pasantes-Morales
Nov 27, 2001·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Alexander A. Mongin, Sergei N. Orlov
Mar 17, 1999·British Journal of Pharmacology·C MaertensB Nilius
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·J VriensB Nilius
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·T VoetsB Nilius
Jan 9, 2010·The Journal of Biological Chemistry·Diego VarelaAndrés Stutzin
Oct 31, 2007·The Journal of General Physiology·Li-Ting Chien, H Criss Hartzell
Nov 27, 2004·The Journal of Physiology·Rodolphe Fischmeister, H Criss Hartzell
Oct 23, 1997·The Journal of Physiology·E Carpenter, C Peers
Nov 1, 2016·Pflügers Archiv : European journal of physiology·Olga PonomarchukRyszard Grygorczyk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.