Activation of thyroid adenyl cyclase by antisera to thyroid plasma membrane preparations: effects of IgG and non-IgG antiserum components

Metabolism: Clinical and Experimental
M OngA Malkin

Abstract

We have previously shown that IgG isolated from rabbit antibovine thyroid plasma membrane (anti-BTPM) antibodies exhibits properties similar to long acting thyroid stimulator (LATS) and HTS-lg in that it activates thyroid adenyl cyclase. In order to test whether another immunoglobulin class, eg, IgM, of anti-BTPM antiserum can also stimulate the bovine thyroid adenyl cyclase system, protein molecules of the antiserum were separated into different molecular sizes by gel filtration chromatography on Sephadex G-200. It was observed that the low molecular weight fraction, consisting predominantly of albumin, was inactive in stimulating adenyl cyclase of the thyroid gland. In contrast, both IgM-enriched and IgG-enriched fractions of the immune serum were fully active. Furthermore, the thyroid-stimulating activity of the IgM-enriched fraction can only be inhibited by anti-IgM and that of the IgG-enriched fraction by anti-IgG. Our data suggest that IgM, in addition to IgG, may also have LATS or LATS-like activities in terms of activating adenyl cyclase of the thyroid gland.

References

Nov 1, 1977·The American Journal of Medicine·A E Kalderon, H A Bogaars
Aug 31, 1972·The New England Journal of Medicine·S C WernerK C Hsu
Feb 1, 1967·The Journal of Clinical Endocrinology and Metabolism·D D Adams, T H Kennedy
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Aug 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·J C MEEKW P VANDERLAAN

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