Abstract
Calcific aortic valve disease (CAVD) is characterized by chronic inflammation and progressive calcification in valve leaflets. Aortic valve interstitial cells (AVICs) play a critical role in the pathogenesis of CAVD. Previous studies show that stimulation of Toll-like receptor (TLR) 2 or TLR4 in AVICs in vitro up-regulates the expression of osteogenic mediators. Double-stranded RNA (dsRNA) can activate pro-inflammatory signaling through TLR3, the NLRP3 inflammasome and RIG-I-like receptors. The objective of this study is to determine the effect of dsRNA on AVIC osteogenic activities and the mechanism of its action. AVICs isolated from normal human valves were exposed to polyinosinic-polycytidylic acid [poly(I:C)], a mimic of dsRNA. Treatment with poly(I:C) increased the production of bone morphogenetic protein-2 (BMP-2), transforming growth factor beta-1 (TGF-β1) and alkaline phosphatase (ALP), and resulted in calcium deposit formation. Poly(I:C) induced the phosphorylation of NF-κB and ERK1/2. Knockdown of TLR3 essentially abrogated NF-κB and ERK1/2 phosphorylation, and markedly reduced the effect of poly(I:C) on the production of BMP-2, TGF-β1 and ALP. Further, inhibition of either NF-κB or ERK1/2 markedly reduced the levels ...Continue Reading
Citations
Feb 3, 2016·Basic Research in Cardiology·Cristiano SpadaccioAlberto Rainer
Sep 21, 2015·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Matthew TorrePaul A VanderLaan
Jul 1, 2015·Biochimica Et Biophysica Acta·Qingzhou YaoXianzhong Meng
Jun 8, 2017·Journal of Biomedical Materials Research. Part a·Sudip DahalGretchen J Mahler
Jun 2, 2016·Journal of Cardiovascular Pharmacology and Therapeutics·Wenguang WangHongliang Cong
Dec 31, 2015·Inflammatory Bowel Diseases·Murtaza M Tambuwala
Apr 26, 2017·Journal of the American Heart Association·Rui SongXianzhong Meng
Apr 1, 2017·Molecular Medicine·Qiong ZhanXianzhong Meng
Dec 12, 2018·Animal Cells and Systems·Seung Hyun Lee, Jae-Hoon Choi
Mar 13, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Philip Roger GoodyFelix Jansen
Mar 30, 2018·Frontiers in Physiology·Carmen García-RodríguezMariano Sánchez Crespo
Mar 30, 2018·Frontiers in Cardiovascular Medicine·Alexia HulinCécile Oury
Sep 11, 2019·European Heart Journal·Silvia Mas-PeiroAndreas M Zeiher
Apr 26, 2017·BMC Immunology·Kazuhide TakadaSatoshi Hayakawa
Nov 21, 2020·Inflammation·Chenyang YuQiang Zheng
Feb 9, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrea Gonzalez RodriguezKristi S Anseth
Nov 3, 2020·Therapeutic Advances in Chronic Disease·Qingchun ZengDingli Xu
May 20, 2021·The FEBS Journal·Iván Parra-IzquierdoCarmen García-Rodríguez
Jul 3, 2021·Biomedicines·Huili LyuDaniel S Perrien
Aug 10, 2021·Journal of Molecular and Cellular Cardiology·Abraham L Bayer, Pilar Alcaide
Apr 27, 2021·The Annals of Thoracic Surgery·Neil VenardosDavid A Fullerton