Activation of Toll-like receptor 2 induces B1 and B2 kinin receptors in human gingival fibroblasts and in mouse gingiva

Scientific Reports
Pedro P C SouzaU H Lerner

Abstract

The regulation of the kallikrein-kinin system is an important mechanism controlling vasodilation and promoting inflammation. We aimed to investigate the role of Toll-like receptor 2 (TLR2) in regulating kinin B1 and B2 receptor expression in human gingival fibroblasts and in mouse gingiva. Both P. gingivalis LPS and the synthetic TLR2 agonist Pam2CSK4 increased kinin receptor transcripts. Silencing of TLR2, but not of TLR4, inhibited the induction of kinin receptor transcripts by both P. gingivalis LPS and Pam2CSK4. Human gingival fibroblasts (HGF) exposed to Pam2CSK4 increased binding sites for bradykinin (BK, B2 receptor agonist) and des-Arg10-Lys-bradykinin (DALBK, B1 receptor agonist). Pre-treatment of HGF for 24 h with Pam2CSK4 resulted in increased PGE2 release in response to BK and DALBK. The increase of B1 and B2 receptor transcripts by P. gingivalis LPS was not blocked by IL-1β neutralizing antibody; TNF-α blocking antibody did not affect B1 receptor up-regulation, but partially blocked increase of B2 receptor mRNA. Injection of P. gingivalis LPS in mouse gingiva induced an increase of B1 and B2 receptor mRNA. These data show that activation of TLR2 in human gingival fibroblasts as well as in mouse gingival tissue lead...Continue Reading

References

Apr 28, 2000·Journal of Clinical Periodontology·F MercadoP M Bartold
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M HirschfeldJ J Weis
Dec 12, 2001·Regulatory Peptides·Anna Bernhold Brechter, Ulf H Lerner
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giselle F PassosJoão B Calixto
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elia BurnsGabriel Nussbaum
Feb 23, 2007·Journal of Dental Research·A Uehara, H Takada
Jun 7, 2007·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·Mhd Khaldoun Al-KatmaAli D Askari
Apr 2, 2009·Nature Reviews. Rheumatology·Paola de PabloThomas Dietrich
Jun 30, 2009·The Journal of Pharmacology and Experimental Therapeutics·Fabiana N DornellesMaria M Campos
Aug 19, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ana Carolina MonteiroJulio Scharfstein
Sep 8, 2009·Journal of Dental Research·L KocgozluS Werner
Nov 26, 2009·Journal of Clinical Periodontology·Rita E Martinez-MartinezJuan Pablo Loyola-Rodríguez
Dec 19, 2009·Periodontology 2000·Yen-Chun G LiuYen-Tung A Teng
May 28, 2010·Journal of Dental Research·P P C SouzaU H Lerner
Feb 26, 2011·Journal of Clinical Periodontology·Gabriel Nussbaum, Lior Shapira
Oct 11, 2011·International Immunopharmacology·Gloria Gutiérrez-Venegas, Juan Antonio Arreguín-Cano
Jul 10, 2012·Journal of Dental Research·R P DarveauM A Curtis
Dec 25, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·George PapadopoulosCaroline A Genco
Feb 25, 2014·Clinical Science·Claudio M Costa-NetoLucas T Parreiras-E-Silva
Apr 1, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sabrina G de AquinoWim B van den Berg
Jun 14, 2014·British Journal of Pharmacology·K OmoriT Murata

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Citations

Sep 2, 2020·Pharmaceuticals·Ana Paula A DagninoRodrigo B M Silva

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BETA
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