Activation of Toll-like receptor 3 increases mouse aortic vascular smooth muscle cell contractility through ERK1/2 pathway

Pflügers Archiv : European journal of physiology
Trevor HardiganMaria Alicia Carrillo-Sepulveda

Abstract

Activation of Toll-like receptor 3 (TLR3), a pattern recognition receptor of the innate immune system, is associated with vascular complications. However, whether activation of TLR3 alters vascular contractility is unknown. We, therefore, hypothesized that TLR3 activation augments vascular contractility and activates vascular smooth muscle cell (VSMC) contractile apparatus proteins. Male mice were treated with polyinosinic-polycytidylic acid (Poly I:C group, 14 days), a TLR3 agonist; control mice received saline (vehicle, 14 days). At the end of protocol, blood pressure was measured by tail cuff method. Aortas were isolated and assessed for contractility experiments using a wire myograph. Aortic protein content was used to determine phosphorylated/total interferon regulatory factor 3 (IRF3), a downstream target of TLR3 signaling, and ERK1/2 using Western blot. We investigated the TLR3/IRF3/ERK1/2 signaling pathway and contractile-related proteins such as phosphorylated/total myosin light chain (MLC) and caldesmon (CaD) in aortic VSMC primary cultures. Poly I:C-treated mice exhibited (vs. vehicle-treated mice) (1) elevated systolic blood pressure. Moreover, Poly I:C treatment (2) enhanced aortic phenylephrine-induced maximum con...Continue Reading

References

Jan 15, 1999·Acta Physiologica Scandinavica·E Takahashi, B C Berk
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masahiro YamamotoShizuo Akira
Sep 15, 2005·Journal of Cardiovascular Pharmacology·A Elizabeth LinderR Clinton Webb
Jun 20, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Xin YangDebbie Beasley
Jul 29, 2008·Investigative Ophthalmology & Visual Science·Yang LiuTeruo Nishida
Feb 18, 2009·Acta Physiologica·P M VanhoutteM Feletou
Mar 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Takayuki MatsumotoKatsuo Kamata
Sep 26, 2009·American Journal of Hypertension·John H TinsleyBrett M Mitchell
Apr 20, 2010·Molecular and Cellular Biochemistry·M A Carrillo-Sepúlveda, M L M Barreto-Chaves
Jul 17, 2010·Pflügers Archiv : European journal of physiology·Matthias Barton
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jennifer E ColeClaudia Monaco
Feb 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Göran K Hansson, Anna M Lundberg
Apr 5, 2011·Journal of the American Society of Hypertension : JASH·Hyehun ChoiR Clinton Webb
May 10, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Takayuki MatsumotoR Clinton Webb
Jun 22, 2011·Acta Physiologica·E Storkebaum, P Carmeliet
Aug 11, 2011·Journal of Vascular Research·Maria Tereza JordãoLisete C Michelini
Apr 3, 2012·Cellular & Molecular Immunology·Monika MerkleMarkus Wörnle
May 5, 2012·Current Hypertension Reports·Michel E SafarAlbert Mimran
May 12, 2012·Cardiology Research and Practice·Rolf SpirigSidney Shaw
May 23, 2012·Metabolism: Clinical and Experimental·Linda H WuVedrana S Susulic
Dec 12, 2012·Acta Physiologica·P SvenningsenB L Jensen
Dec 25, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Takuji IshimotoRichard J Johnson
Jan 9, 2013·Journal of Innate Immunity·Michifumi YamashitaSteven N Emancipator
May 3, 2013·PloS One·Maria Alícia Carrillo-SepúlvedaMaria Luiza M Barreto-Chaves
Aug 21, 2013·Nature Reviews. Immunology·J Simon C Arthur, Steven C Ley
Jan 11, 2014·European Journal of Endocrinology·Taís Silveira AssmannDaisy Crispim
Apr 23, 2014·Hypertension·Gary F Mitchell
Sep 27, 2014·Hypertension·Daniel W TrottDavid G Harrison

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