Activation of Toll-like receptor 5 decreases the attachment of human trophoblast cells to endometrial cells in vitro

Human Reproduction
Wedad AboussahoudAlireza Fazeli

Abstract

Embryo implantation in the uterus involves the trophoblast cells apposing and adhering to, then invading across the epithelium lining of the endometrium. However, ethical concerns regarding experimentation with primary human tissue during this period of life necessitates creation of in vitro models for understanding the basic mechanisms involved. Toll-like receptors (TLRs) play a crucial role in defence against pathogens invading the female reproductive tract. The objective of this study is to establish and optimize an in vitro model for studying human endometrial embryonic interactions and to understand the effect of TLR5 stimulation on the attachment of trophoblast cells to endometrial cells. By using a human telomerase immortalized endometrial epithelial cell line (hTERT-EECs) and choriocarcinoma human trophoblast cells (JAr cells), an in vitro assay of human implantation was established. In order to investigate the impact of TLR5 stimulation on attachment in this assay, bacterial flagellin was applied to the endometrial and trophoblast cells. In order to block TLR5 in the endometrial and trophoblast cells, TLR5 function-blocking antibody was applied to the cells prior to flagellin treatment. The results demonstrated that JA...Continue Reading

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Citations

Oct 29, 2013·Reproductive Biomedicine Online·Charlotte H E WeimarNick S Macklon
Sep 24, 2013·Reproductive Biomedicine Online·Charlotte H E WeimarNick S Macklon
Jan 15, 2014·Molecular Reproduction and Development·Sonali R BhagwatGeetanjali Sachdeva
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Nov 1, 2015·Biochemical Society Transactions·Shaghayegh BasatvatAlireza Fazeli
Apr 10, 2019·The Journal of Experimental Medicine·Seunghee HongVirginia Pascual
Jan 8, 2021·Human Reproduction Update·Samuel OjosnegrosAnna Veiga
Jun 19, 2021·Communications Biology·Sophie BergmannThorsten E Boroviak

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