Activation of transient receptor potential vanilloid 4 channels dilates rat retinal arterioles through nitric oxide- and BKCa channel-dependent mechanisms in vivo

Naunyn-Schmiedeberg's Archives of Pharmacology
Asami MoriTsutomu Nakahara

Abstract

Transient receptor potential vanilloid 4 (TRPV4) channel, a cation channel expressed in nearly all cell types, plays an important role in the regulation of vascular tone. In the present study, we examined the effect of GSK1016790A, an activator of TRPV4 channels, on the diameter of retinal blood vessels in rats and the underlying mechanisms. Ocular fundus images were captured with an original high-resolution digital fundus camera in vivo and diameters of retinal blood vessels were measured. Intravenous infusion of GSK1016790A (0.2-2 μg kg-1 min-1) increased retinal arteriolar diameter in a dose-dependent manner. The higher dose of GSK1016790A (2 μg kg-1 min-1) slightly decreased blood pressure. These responses to GSK1016790A were significantly attenuated by intravenous injection of GSK2193874 (0.3 mg/kg), an antagonist of TRPV4 channels. Intravitreal injection of Nω-nitro-L-arginine methyl ester, an inhibitor of nitric oxide (NO) synthase or iberiotoxin, an inhibitor of large-conductance Ca2+-activated K+ (BKCa) channel, significantly attenuated the GSK1016790A-induced increases in retinal arteriolar diameter. These results suggest that activation of TRPV4 channels dilates rat retinal arterioles through NO- and BKCa channel-dep...Continue Reading

References

Oct 3, 2000·Ophthalmic Research·C Delaey, J Van De Voorde
May 6, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Ralf KöhlerJoachim Hoyer
May 2, 2008·Progress in Retinal and Eye Research·Constantin J PournarasEinar Stefansson
Aug 4, 2009·Experimental Eye Research·Shanti R TummalaJennifer J Kang Derwent
Dec 8, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Suelhem A MendozaDavid X Zhang
Mar 13, 2010·Pflügers Archiv : European journal of physiology·William B Campbell, Ingrid Fleming
Oct 28, 2010·Naunyn-Schmiedeberg's Archives of Pharmacology·Asami MoriKunio Ishii
May 13, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel A RyskampDavid Krizaj
Oct 31, 2012·Journal of Cardiovascular Pharmacology·Jessica A FilosaGeraldine Rath
Sep 27, 2013·Journal of Pharmacological Sciences·Tsutomu NakaharaKunio Ishii
Nov 5, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Edward A PankeyPhilip J Kadowitz
May 27, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Eric A Newman
Jul 8, 2015·European Journal of Pharmacology·Asami MoriKunio Ishii
Oct 2, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andrew O JoDavid Križaj
Oct 27, 2016·Investigative Ophthalmology & Visual Science·Mary K McGahonTim M Curtis

❮ Previous
Next ❯

Citations

Jan 5, 2021·Biological & Pharmaceutical Bulletin·Asami MoriTsutomu Nakahara
May 14, 2021·Frontiers in Molecular Biosciences·Liangliang LiuTingting Zhou

❮ Previous
Next ❯

Software Mentioned

PRISM6
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.