Activation of TRP channels by protons and phosphoinositide depletion in Drosophila photoreceptors.

Current Biology : CB
Jiehong HuangRoger C Hardie

Abstract

Phototransduction in microvillar photoreceptors is mediated via G protein-coupled phospholipase C (PLC), but how PLC activation leads to the opening of the light-sensitive TRPC channels (TRP and TRPL) remains unresolved. In Drosophila, InsP(3) appears not to be involved, and recent studies have implicated lipid products of PLC activity, e.g., diacylglycerol, its metabolites, or the reduction in PIP(2). The fact that hydrolysis of the phosphodiester bond in PIP(2) by PLC also releases a proton is seldom recognized and has neither been measured in vivo nor implicated previously in a signaling context. Following depletion of PIP(2) and other phosphoinositides by a variety of experimental manipulations, the light-sensitive channels in Drosophila photoreceptors become remarkably sensitive to rapid and reversible activation by the lipophilic protonophore 2-4 dinitrophenol in a pH-dependent manner. We further show that light induces a rapid (<10 ms) acidification originating in the microvilli, which is eliminated in mutants of PLC, and that heterologously expressed TRPL channels are activated by acidification of the cytosolic surface of inside-out patches. Our results indicate that a combination of phosphoinositide depletion and acidi...Continue Reading

References

Jul 1, 1980·Physiological Reviews·S G McLaughlin, J P Dilger
Mar 1, 1996·The Journal of Biological Chemistry·M T PearnW L Pak
Jun 2, 2000·Molecular and Cellular Neurosciences·P RaghuR C Hardie
Jun 5, 2001·Nature Reviews. Neuroscience·D E ClaphamC Strübing
Jan 16, 2002·The Journal of Physiology·Christof J Schwiening, Debbie Willoughby
Feb 25, 2005·Science's STKE : Signal Transduction Knowledge Environment·Craig Montell
Jul 12, 2005·Current Biology : CB·Yuchun GuRoger C Hardie
Jan 20, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Che H LiuRoger C Hardie
May 5, 2007·Pflügers Archiv : European journal of physiology·Tibor Rohacs, Bernd Nilius
May 10, 2007·Pflügers Archiv : European journal of physiology·Tao Wang, Craig Montell
Sep 22, 2007·The Journal of Biological Chemistry·Marcus SemtnerTim D Plant
Jan 31, 2008·The Journal of Biological Chemistry·Ken-ichi OtsuguroAlexander V Zholos
Jul 31, 2008·Pflügers Archiv : European journal of physiology·Mohamed TrebakJames W Putney
Feb 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Moshe ParnasBaruch Minke
Apr 14, 2009·Cell Calcium·Padinjat Raghu, Roger C Hardie
Jul 23, 2009·Frontiers in Cellular Neuroscience·Ben Katz, Baruch Minke
Aug 6, 2009·Handbook of Experimental Pharmacology·Peter Holzer
Nov 18, 2009·The Journal of General Physiology·Jianyang DuLixia Yue
Dec 1, 1972·The Journal of Membrane Biology·S McLaughlin

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Citations

Aug 7, 2013·Advances in Biological Regulation·Tibor Rohacs
Oct 11, 2011·Nature·Daniel VasiliauskasClaude Desplan
Jul 29, 2011·The Journal of Biological Chemistry·Melissa MillerMichael X Zhu
May 5, 2012·Journal of Biochemistry·Seong Eon Ryu
Oct 16, 2012·Science·Roger C Hardie, Kristian Franze
Oct 9, 2013·Annual Review of Cell and Developmental Biology·David Julius
Jun 12, 2013·Annual Review of Neuroscience·Niels EijkelkampJohn N Wood
Feb 8, 2011·Human Genomics·Zan PanPeter S Reinach
Dec 7, 2013·PloS One·Peter M ZygmuntEdward D Högestätt
May 30, 2014·PloS One·Kylie S ChewJeffrey M Trimarchi
Oct 1, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yuanyuan Y WangEmily R Liman
Feb 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ben Katz, Baruch Minke
May 10, 2011·Annual Review of Biochemistry·Silvia Carrasco, Tobias Meyer
Apr 11, 2014·Cells·Olaf Voolstra, Armin Huber
May 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Andriy AnishkinChing Kung
Dec 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Wenlei YeEmily R Liman
Aug 11, 2012·Life Sciences·Melissa A Fowler, Craig Montell
Apr 14, 2012·Trends in Neurosciences·Craig Montell
Apr 11, 2012·Biochimica Et Biophysica Acta·Padinjat RaghuNaresh Babu Naidu Mallampati
Sep 22, 2011·Advances in Biological Regulation·James W Putney, Takuro Tomita
May 27, 2016·Temperature : Multidisciplinary Biomedical Journal·Maristela O PoletiniAna Maria de Lauro Castrucci
Mar 19, 2011·EMBO Reports·Stefan G Lechner, Jan Siemens
Jun 30, 2012·Journal of Thrombosis and Haemostasis : JTH·M P Mahaut-Smith
Feb 2, 2015·Current Opinion in Neurobiology·Roger C Hardie, Mikko Juusola
Jun 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Gabriel PeinadoEnrico Nasi
Jun 13, 2015·European Biophysics Journal : EBJ·Peihua ChenXiao-dong Liu
Jan 31, 2012·Journal of Neurogenetics·Randall D Shortridge
Sep 18, 2013·Biochimica Et Biophysica Acta·Mitsumasa Koyanagi, Akihisa Terakita
Nov 22, 2011·Neuron·Erica E RosenbaumNansi Jo Colley
Jan 12, 2013·Current Biology : CB·Nerissa K Kirkwood, Joerg T Albert
Feb 25, 2010·Current Biology : CB·Thomas Gudermann, Michael Mederos y Schnitzler
Sep 10, 2013·Trends in Neurosciences·Bo Xiong, Hugo J Bellen

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