Activation of Wnt signalling reduces the population of cancer stem cells in ameloblastoma.

Cell Proliferation
Hyun-Yi KimHan-Sung Jung

Abstract

The treatment of ameloblastoma, an odontogenic epithelial tumour destroying jawbone, mainly depends on radical destructive resections. Other therapeutic options are limited by the characteristics of ameloblastoma, such as high recurrence rates and resistance to radiation and chemotherapy, which implies possible existence of cancer stem cells (CSCs) in ameloblastoma. Here, we identified a putative CSC population in immortalized and primary human ameloblastoma cells and examined possible therapeutic reagents to reduce the CSC population. We investigated subpopulations of AM-1 cell line and human ameloblastoma cells using immunocytochemistry and flow cytometry and the effects of Wnt signalling activators on the 2- and 3-dimensional cultured ameloblastoma cells using molecular biological analyses. Among heterogenous ameloblastoma cells, small-sized and round-shaped cells were found to be proliferative and expressed a marker of dental epithelial stem cells, SRY-box 2 (Sox2). Exogenous activation of Wnt signalling using glycogen synthase kinase 3β inhibitors, lithium chloride (LiCl) and valproic acid (VPA), increased the cell size and decreased proliferation of cells and expression of Sox2 in 2 dimensionally cultured AM-1 and human p...Continue Reading

References

Jul 31, 1998·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·H HaradaS Yasumoto
Mar 29, 2005·Oral Oncology·Laifa HendarminNorifumi Nakamura
Aug 8, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Hiroyuki Kumamoto, Kousuke Ohki
Nov 3, 2009·ANZ Journal of Surgery·Eric ShamAlan K Mansour
Nov 15, 2011·Gene·David G MonroeJennifer J Westendorf
Nov 20, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Gulsan A SathiHitoshi Nagatsuka
Jul 10, 2013·International Journal of Oncology·Shiori KandaSeiji Nakamura
Aug 3, 2013·Stem Cell Research·Julia Yu Fong ChangFen Wang
May 27, 2014·Nature Genetics·Robert T SweeneyRobert B West
Jul 6, 2014·Advanced Drug Delivery Reviews·Gabriel BentonJennifer Koblinski
May 1, 2015·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Andrew C McClaryDavud Sirjani
Jan 23, 2016·International Journal of Oncology·Shohei YoshimotoMasato Hirata
Feb 6, 2016·Cell and Tissue Research·Min-Jung LeeHan-Sung Jung
Mar 10, 2016·European Journal of Oral Sciences·Xiaomu GuanJohn D Bartlett
Apr 12, 2016·Pharmacology & Therapeutics·Sritama Nath, Gayathri R Devi
Jun 16, 2016·Frontiers in Physiology·Sujeenthar Tharmalingam, David R Hampson
Jun 22, 2016·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Yuichiro TakebeHitoshi Nagatsuka
Jul 20, 2016·Cancer Treatment Reviews·A CarneroM E LLeonart
Sep 28, 2016·International Journal of Cancer. Journal International Du Cancer·Gustav van NiekerkAnna-Mart Engelbrecht
Oct 5, 2017·Therapeutic Advances in Musculoskeletal Disease·Michael R McClung
Mar 10, 2018·Head & Neck·Matthew TamplenTamara Alliston
Jun 5, 2019·Nature Reviews. Drug Discovery·Asher Mullard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Otolaryngologia polska. The Polish otolaryngology
J Kubiczkowa
Nederlands tijdschrift voor tandheelkunde
M S CuneL E Smeele
Journal of the Indian Dental Association
S S Sidhu, G L Subherwal
The Journal of the Tennessee Dental Association
J M McCoy, W R High
Nederlands tijdschrift voor geneeskunde
A N GeelI van der Waal
© 2021 Meta ULC. All rights reserved