PMID: 7514808May 1, 1994Paper

Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells

Scandinavian Journal of Immunology
Y Watanabe, T Akaike

Abstract

T lymphocytes express multiple forms of the leukocyte-common antigen CD45, transcribed by alternative usage of leukocyte-common antigen exon 4-6. The various isoforms of CD45R expressed differentially on T cells are involved in different stages of development and activation. The monoclonal antibody (MoAb) RA3-6B2 is established as a B cell-type isoform (B220)-specific marker. However, it reacts with certain activated T cells although the relationship between 6B2 expression and T-cell activation is unclear. We have examined the 6B2 expression on activated T cells and found that concanavalin A, anti-CD3 antibody and staphylococcal enterotoxin B (SEB) induced 6B2 expression on T cells. The expression was found on both CD4+ and CD8+ T cells and also was induced by SEB in vivo predominantly on CD8+ T cells. The 6B2+ T cells are IL-2R+ and blasted cells according to flow cytometry analysis. Therefore, the 6B2+ T cells are supposed to be in an activated stage. Enzymatic analysis demonstrated that trypsin treatment decreased the 6B2 expression, whereas neuraminidase increased the intensity on activated T cells. Neither endo-D or endo-H have any effect on the expression and there are no differences, in the results of immunoprecipitation...Continue Reading

References

Jun 1, 1992·European Journal of Immunology·W T Lee, E S Vitetta
Jul 1, 1992·European Journal of Immunology·Y FujiiH Matsuda
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G A KoretzkyA Weiss
Oct 1, 1990·The Journal of Experimental Medicine·Y Kawabe, A Ochi
Oct 11, 1990·European Journal of Immunology·U DianzaniK Bottomly
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H L OstergaardI S Trowbridge
Dec 1, 1989·The Journal of Experimental Medicine·S A BrodD A Hafler
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·O LeoJ A Bluestone
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M L ThomasI S Trowbridge
Oct 1, 1988·Immunology Today·M L Thomas, L Lefrançois
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y SagaE A Boyse

❮ Previous
Next ❯

Citations

Nov 26, 2009·The Journal of Experimental Medicine·Tomonori HosoyaJames Douglas Engel
Sep 17, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·P B ErnstK S Tung
Feb 21, 2002·Biological & Pharmaceutical Bulletin·Katsunori YamauraKoichi Ueno
Aug 21, 2010·Journal of Medical Microbiology·Ema PaulovičováSlavomír Bystrický
May 26, 1999·The Journal of Veterinary Medical Science·S OkaY Takamori
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martina Johansson, Nils Lycke
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amedeo J CappioneIñaki Sanz
Jun 30, 1998·European Journal of Immunology·L CederbomF Ivars

❮ Previous
Next ❯