Activation signals in human lymphocytes: interleukin 2 synthesis and expression of high affinity interleukin 2 receptors require differential signalling for the activation of protein kinase C

Cellular Immunology
M SzamelK Resch

Abstract

The mitogenic activation of resting T lymphocytes involves two distinct cellular events, the synthesis of the ultimate mitogen interleukin 2 and the synthesis and expression of receptors for it. In order to get more detailed information on the mechanisms associated with these activating steps (the effects of different stimuli, leading to activation of protein kinase C were investigated in human lymphocytes). The anti-T-cell receptor (TCR) and anti-CD3 monoclonal antibodies (BMA 031 and BMA 030, respectively), as well as the combination of the phorbol ester, TPA, with a calcium ionophore-induced interleukin 2 synthesis and subsequent proliferation in human peripheral blood lymphocytes. Incubation of cells with synthetic diacylglycerols and calcium ionophores proved to be effective in expression of high affinity interleukin receptors, no detectable amounts of interleukin 2 were, however, synthetized. When diacylglycerols were, however, added repetitively, interleukin 2 was also produced. Both anti-TCR/CD3 antibodies and TPA or DiC8 caused activation and translocation of protein kinase C from the cytosol to the plasma membrane. Significant differences, however, were observed between the time kinetics of the translocation of the en...Continue Reading

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Citations

Mar 4, 1999·European Journal of Immunology·M A López-LagoP Barja
Jul 1, 1993·Journal of Reproductive Immunology·E J BlumenthalR C Hoversland
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y AsaokaY Nishizuka
Jul 1, 1996·The Journal of Experimental Medicine·J A Garcia-Sanz, D Lenig
Aug 15, 1991·Biochemical and Biophysical Research Communications·Y AsaokaY Nishizuka
Nov 1, 1991·Clinical and Experimental Immunology·K YonedaT Osaki

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