PMID: 6973007Jul 1, 1981Paper

Active and passive electrical properties of single bullfrog atrial cells

The Journal of General Physiology
J R Hume, W R Giles

Abstract

Single cells from the bullfrog (Rana catesbeiana) atrium have been prepared by using a modification of the enzymatic dispersion procedure described by Bagby et al. (1971. Nature [Long.]. 234:351--352) and Fay and Delise (1973. Proc. Natl. Acad. Sci. U.S.A. 70:641--645). Visualization of relaxed cells via phase-contrast or Nomarski optics (magnification, 400--600) indicates that cells range between 150 and 350 micrometers in length and 4 and 7 micrometers in diameter. The mean sarcomere length in relaxed, quiescent atrial cells in 2.05 micrometer. Conventional electrophysiological measurements have been made. In normal Ringer's solution (2.5 mM K+, 2.5 mM Ca++) acceptable cells have stable resting potentials of about -88 mV, and large (125 mV) long-duration (approximately 720 ms) action potentials can be elicited. The Vm vs. log[K+]0 relation obtained from isolated cells is similar to that of the intact atrium. The depolarizing phase of the action potential of isolated atrial myocytes exhibits two pharmacologically separable components: tetrodotoxin (10(-6) g/ml) markedly suppresses the initial regenerative depolarization, whereas verapamil (3 x 10(-6) M) inhibits the secondary depolarization and reduce the plateau height. A bri...Continue Reading

References

Apr 1, 1979·Journal of Molecular and Cellular Cardiology·G V VahounyE N Albert
Mar 1, 1977·Archives of Biochemistry and Biophysics·B B FarmerH R Besch
Aug 1, 1979·Quarterly Reviews of Biophysics·D AttwellI Cohen
Jan 1, 1977·Progress in Biophysics and Molecular Biology·D Attwell, I Cohen
Jan 1, 1977·Annual Review of Pharmacology and Toxicology·A Fleckenstein
Jan 1, 1979·Annual Review of Physiology·H Reuter
Jan 1, 1979·Annual Review of Biophysics and Bioengineering·S Hagiwara, L A Jaffe
Mar 15, 1979·Nature·K S LeeA M Brown
Dec 1, 1977·The Journal of General Physiology·D C Gadsby, P F Cranefield
Aug 1, 1978·Experimental Cell Research·J RajsS W Jakobsson
Sep 7, 1976·Biochemical and Biophysical Research Communications·Y IkeharaK Kato
Oct 1, 1975·Biophysical Journal·J ConnorE Jakobsson
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·F S Fay, C M Delise
Sep 1, 1974·Analytical Biochemistry·M R GlickW J Reddy
Sep 1, 1974·The Journal of General Physiology·S Winegrad
Jan 1, 1971·Annual Review of Physiology·E A Johnson, M Lieberman
Mar 1, 1969·Circulation Research·W C de MelloM Chapeau
Feb 1, 1972·The Journal of General Physiology·P F CranefieldB F Hoffman
Jan 1, 1971·Pflügers Archiv : European journal of physiology·H G HaasM Tarr
Nov 1, 1971·The Journal of General Physiology·M Tarr, J Trank
Jan 1, 1971·Pflügers Archiv : European journal of physiology·A De Hemptinne
Dec 1, 1969·The Journal of General Physiology·Y Sakamoto
Jun 1, 1970·The Journal of General Physiology·R S Eisenberg, E Engel
Sep 1, 1970·The Journal of Cell Biology·K M Baldwin
May 15, 1980·Experientia·A I UndrovinasL V Rosenshtraukh
Feb 2, 1965·Naunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie·H G GLITSCHW TRAUTWEIN
May 1, 1965·The Journal of General Physiology·L BARRW BERGER

❮ Previous
Next ❯

Citations

Apr 1, 1993·Pflügers Archiv : European journal of physiology·G CecchiC Tesi
Aug 1, 1984·Naunyn-Schmiedeberg's Archives of Pharmacology·M NodaM Fujiwara
Oct 1, 1985·Pflügers Archiv : European journal of physiology·L Tung, M Morad
Dec 1, 1983·Pflügers Archiv : European journal of physiology·H G HaasW Stockem
Jan 1, 1984·The Journal of Membrane Biology·L E MooreG Isenberg
Dec 12, 1994·European Journal of Pharmacology·Q HuangY Momose
Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·P Karttunen, R Tirri
Jan 1, 1987·Comparative Biochemistry and Physiology. A, Comparative Physiology·P Karttunen, R Tirri
Jul 1, 1986·Journal of the American College of Cardiology·H A FozzardM F Sheets
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E F Shibata, W R Giles
Nov 1, 1987·The Journal of General Physiology·G N TsengB F Hoffman
Jan 1, 1988·The Journal of General Physiology·D D Friel, B P Bean
Apr 1, 1988·The Journal of General Physiology·R D NathanW Giles
Jan 1, 1990·Annals of the New York Academy of Sciences·J P Keener
Oct 17, 1981·British Medical Journal·T PowellV W Twist
Aug 1, 1985·IEEE Transactions on Bio-medical Engineering·N GanapathyW Giles
Oct 1, 1983·Circulation Research·M F SheetsH A Fozzard
Apr 1, 1985·Circulation Research·H A FozzardJ C Makielski
Oct 1, 1986·Circulation Research·M TarrK K Goertz
Mar 1, 1983·Circulation Research·T WatanabeT F McDonald
Feb 1, 1984·Circulation Research·I R JosephsonA M Brown
Jul 26, 2003·American Journal of Physiology. Heart and Circulatory Physiology·R A RoseW R Giles
Dec 9, 1998·The American Journal of Physiology·L Hove-Madsen, L Tort
Sep 1, 1987·The American Journal of Physiology·M LiebermanM Poenie
Aug 1, 1986·The American Journal of Physiology·M Désilets, C M Baumgarten
Feb 1, 1989·The American Journal of Physiology·K W Spitzer, J H Bridge
Nov 1, 1986·The American Journal of Physiology·N Shepherd, F Kavaler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.