Active and secreted IgA-coated bacterial fractions from the human gut reveal an under-represented microbiota core

Scientific Reports
Giuseppe D'AuriaAndrés Moya

Abstract

Host-associated microbiota varies in distribution depending on the body area inhabited. Gut microbes are known to interact with the human immune system, maintaining gut homoeostasis. Thus, we studied whether secreted-IgA (S-IgA) coat specific microbial taxa without inducing strong immune responses. To do so, we fractionated gut microbiota by flow cytometry. We found that active and S-IgA-coated bacterial fractions were characterized by a higher diversity than those observed in raw faecal suspensions. A long-tail effect was observed in family distribution, revealing that rare bacteria represent up to 20% of total diversity. While Firmicutes was the most abundant phylum, the majority of its sequences were not assigned at the genus level. Finally, the single-cell-based approach enabled us to focus on active and S-IgA-coated bacteria. Thus, we revealed a microbiota core common to the healthy volunteers participating in the study. Interestingly, this core was composed mainly of low frequency taxa (e.g. Sphingomonadaceae).

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Datasets Mentioned

BETA
ERP002046

Methods Mentioned

BETA
flow cytometry
PCR
for

Software Mentioned

Adonis Analysis
Bioconductor
Vennerable R package
R package flowCore
Prinseq
Vegan R package
R
flowViz

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