Active extracts of wild fruiting bodies of Antrodia camphorata (EEAC) induce leukemia HL 60 cells apoptosis partially through histone hypoacetylation and synergistically promote anticancer effect of trichostatin A

Archives of Toxicology
Mei-Chin LuYang-Chang Wu

Abstract

The endemic species of Antrodia camphorate (AC) is a promising chemotherapeutic drug for cancer. We found that the ethanol extract from wild fruiting bodies of Antrodia camphorata (EEAC) could induce HL 60 cells apoptosis via histone hypoacetylation, up-regulation of histone deacetyltransferase 1 (HDAC 1), and down-regulation of histone acetyltransferase activities including GCN 5, CBP and PCAF in dose-dependent manner. In combination with histone deacetylase inhibitor, trichostatin A (TSA), did not block EEAC-induced apoptosis. Interestingly, combined treatment (100 nM of TSA and 100 microg/ml EEAC) caused synergistic inhibition of cell growth and increase of apoptotic induction. EEAC could effectively increase the cytotoxic sensitivity of TSA through the up-regulation of DR5 and NFkappaB activation. In this present study, bioassay-guided fractionation of EEAC led to a major active compound, zhankuic acid A, as the bioactive marker. Moreover, our findings may represent an experimental basis for developing EEAC as a potential chemotherapeutic adjuvant.

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Citations

Nov 5, 2013·Archives of Toxicology·S HammadH M Bolt
Apr 23, 2011·Bioscience, Biotechnology, and Biochemistry·Chia-Yu ChangJiunn-Jye Chuu
Apr 9, 2013·Pharmacology & Therapeutics·Mei-Chin LuYang-Chang Wu
Aug 19, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Madamanchi Geethangili, Yew-Min Tzeng
Aug 9, 2012·World Journal of Microbiology & Biotechnology·Yu-Hsin ChenFu-Wen Kuo
Apr 21, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ying-Chi DuMei-Chin Lu
Sep 19, 2009·Leukemia Research·Ana Lucia AbujamraAlgemir Lunardi Brunetto
Mar 11, 2015·Journal of Cellular Biochemistry·Ann-Christin GaupelMartin Tenniswood
Jul 31, 2014·Biochemical Pharmacology·Yu-Fon ChenTian-Shung Wu
Mar 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi-Ming ChenHsin-Li Liang
Jul 5, 2019·Integrative Cancer Therapies·Yi-Hsuan HsiaoShun-Fa Yang
Jun 19, 2009·Journal of Natural Products·I-Hsiao ChenYang-Chang Wu

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis