Active immunization against nicotine alters the distribution of nicotine but not the metabolism to cotinine in the rat

Naunyn-Schmiedeberg's Archives of Pharmacology
Sabina de VilliersTorgny H Svensson

Abstract

We have previously shown that active immunization with the nicotine immunoconjugate IP18-KLH attenuates the reinforcing effects of nicotine, i.e., suppresses the nicotine-induced brain dopamine release and prevents reinstatement of the nicotine-seeking behavior in rats. These effects are thought to be due to an alteration of the kinetics of nicotine distribution by the antibodies, resulting in an attenuated nicotine distribution to the brain. In this study, the distribution of nicotine administered at doses corresponding to those used in our previous studies, was investigated in immunized rats and controls. Male Wistar rats received two immunizations with IP18-KLH in Freund's incomplete adjuvant, 21 days apart, and experiments were performed 7-11 days post-immunization under chloral hydrate anesthesia. Blood samples were collected to determine antibody titer and nicotine selectivity using enzyme-linked immunosorbent assay (ELISA) techniques. The animals received an intravenous nicotine dose and were sacrificed either 3 min or 60 min after nicotine administration. Trunk blood was collected and the brains were removed for analysis of nicotine content. The results showed that immunization against nicotine increases the nicotine co...Continue Reading

References

May 18, 1999·Psychopharmacology·Y HiedaP R Pentel
Nov 10, 2000·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·D E KeylerP R Pentel
Jul 5, 2002·Respiration; International Review of Thoracic Diseases·Paul Pentel, David Malin
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Jul 5, 2002·Respiration; International Review of Thoracic Diseases·N LindblomT H Svensson

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Citations

Oct 7, 2006·The Journal of Pharmacology and Experimental Therapeutics·Andrew B NormanWilliam J Ball
Dec 18, 2008·The Journal of Pharmacology and Experimental Therapeutics·Andrew B NormanWilliam J Ball
Feb 24, 2012·Drugs·Tobias RaupachConstant P Onno van Schayck
Aug 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eric P ZorrillaKim D Janda
Nov 1, 2007·Expert Opinion on Investigational Drugs·Patrik Maurer, Martin F Bachmann
Jul 29, 2008·International Immunopharmacology·D E KeylerP R Pentel
Nov 15, 2011·Journal of Molecular Biology·Kaspars TarsPatrik Maurer
Jun 30, 2015·Drug and Alcohol Dependence·M L MillerM A Taffe
Jul 5, 2011·Neuroscience and Biobehavioral Reviews·Henri-Jean AubinGeorg Winterer
Feb 29, 2008·The Journal of Pharmacology and Experimental Therapeutics·Samuel A RoikoPaul R Pentel
Jan 29, 2011·Clinical Pharmacology and Therapeutics·D K HatsukamiS I Rennard

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