Active leukocyte crawling in microvessels assessed by digital time-lapse intravital microscopy

The Journal of Surgical Research
Eduard RyschichJan Schmidt

Abstract

The ability of active movement is an important feature of leukocytes. Here, we used a hybrid technique that combines intravital microscopy and digital time-lapse video microscopy to investigate the physiology and molecular mechanisms of intravascular leukocyte movement. Intravital microscopy of mesenteric venules was performed in male, Wistar rats using digital video recording and time-lapse image compression. The leukocyte movement and extravasation were analyzed after local application of TNF-alpha, after blockade of endothelial (anti-ICAM-1 antibody) and leukocyte (anti-CD18 antibody) adhesion molecules. Additionally, the migratory activity of isolated leukocytes in collagen gel was analyzed and compared with their intravascular locomotion. Adherent leukocytes showed an active intraluminal crawling along the endothelial lining. Most permanent stickers (84 +/- 13%) crawled actively on the intraluminal site of venules. Baseline measurement of leukocyte crawling velocity yielded an average 9.0 +/- 1.8 mum/min that was not significantly different from crawling velocity of extravascular leukocytes (8.9 +/- 4.5 mum/min). The maximum distance of leukocyte crawling observed was 150 microm. The maximum time of crawling was 15 min. In...Continue Reading

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Citations

Feb 4, 2010·Cardiovascular Research·Connie H Y WongPaul Kubes
Nov 20, 2009·Current Opinion in Hematology·Christoph ScheiermannPaul S Frenette
Jun 17, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Filip K Swirski
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May 4, 2010·Cellular and Molecular Life Sciences : CMLS·Clinton S Robbins, Filip K Swirski
Jun 4, 2013·The Journal of Surgical Research·Edward Castro-SantaEduard Ryschich
Apr 17, 2015·Thrombosis and Haemostasis·Mehran Ghasemzadeh, Ehteramolsadat Hosseini
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May 1, 2021·Cancers·Yang ZhaoJennifer Gamble
Jul 29, 2021·Drug Delivery and Translational Research·Baifeng QianEduard Ryschich

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