Active-passive calibration of optical tweezers in viscoelastic media

The Review of Scientific Instruments
Mario FischerKirstine Berg-Sørensen

Abstract

In order to use optical tweezers as a force measuring tool inside a viscoelastic medium such as the cytoplasm of a living cell, it is crucial to perform an exact force calibration within the complex medium. This is a nontrivial task, as many of the physical characteristics of the medium and probe, e.g., viscosity, elasticity, shape, and density, are often unknown. Here, we suggest how to calibrate single beam optical tweezers in a complex viscoelastic environment. At the same time, we determine viscoelastic characteristics such as friction retardation spectrum and elastic moduli of the medium. We apply and test a method suggested [M. Fischer and K. Berg-Sørensen, J. Opt. A, Pure Appl. Opt. 9, S239 (2007)], a method which combines passive and active measurements. The method is demonstrated in a simple viscous medium, water, and in a solution of entangled F-actin without cross-linkers.

References

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Citations

Oct 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Adam G HendricksYale E Goldman
Apr 5, 2011·The Review of Scientific Instruments·Scott A SilburnGordon D Love
Jan 7, 2014·The Review of Scientific Instruments·Qili ZhaoXizeng Feng
Sep 21, 2013·Biophysical Journal·Marco Capitanio, Francesco S Pavone
Feb 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Benjamin H BlehmPaul R Selvin
Feb 7, 2017·Journal of Biophotonics·Florian HörnerErez Raz
Feb 22, 2017·Scientific Reports·Frederic CatalàEstela Martín-Badosa
Oct 19, 2012·Nature Chemical Biology·Lene B Oddershede
Mar 22, 2014·Physical Chemistry Chemical Physics : PCCP·Kamilla NorregaardLene B Oddershede
Dec 3, 2017·The Review of Scientific Instruments·Hao YanSimon G J Mochrie
Jan 27, 2019·Scientific Reports·Dipankar MondalDebabrata Goswami
Nov 3, 2020·Frontiers in Molecular Biosciences·Kathrin LehmannNancy R Forde
Jun 3, 2021·Micromachines·Alexander B StilgoeHalina Rubinsztein-Dunlop
Feb 6, 2017·Chemical Reviews·Kamilla NorregaardLene B Oddershede
Oct 15, 2021·Nature Communications·Archishman GhoshHuan-Xiang Zhou
Sep 16, 2014··Jordi Sancho-ParramonArnau Farré

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Methods Mentioned

BETA
force measurements
optical tweezers

Software Mentioned

LABVIEW

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