Active Probes for Imaging Membrane Dynamics of Live Cells with High Spatial and Temporal Resolution over Extended Time Scales and Areas

Journal of the American Chemical Society
Huaimin WangBing Xu

Abstract

Despite the advancement of molecular imaging techniques, there is an unmet need for probes for direct imaging of membrane dynamics of live cells. Here we report a novel type of active (or enzyme responsive) probes to directly image membrane dynamics of live cells with high spatial and temporal resolution over extended time scales and areas. Because lipid rafts enrich cholesterols and GPI-anchored enzymes (e.g., ectophosphatases), we design probes that consist of an enzymatic trigger, a fluorophore, and a cholesterol that are affinitive to the cell membrane. Being water-soluble and as the substrate of ectophosphatase, these cell compatible probes preferentially and rapidly assemble in plasma membrane, exhibit strong fluorescence, work at micromolar concentrations, and easily achieve high resolution monitoring of nanoscale heterogeneity in membranes of live cells, the release of exosomes, and the membrane dynamics of live cells. This work provides a facile means to link membrane dynamics and heterogeneity to cellular processes for understanding the interactions between membranes and proteins.

Citations

Oct 26, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Paltan LahaSrikanta Patra
Nov 6, 2018·Chembiochem : a European Journal of Chemical Biology·Jiaqing WangBing Xu
Mar 1, 2019·Molecular Medicine Reports·Shengcai QiYuanzhi Xu
Sep 10, 2020·Advanced Healthcare Materials·Yu DengGaolin Liang
Apr 9, 2019·Chembiochem : a European Journal of Chemical Biology·Huaimin WangBing Xu
Nov 17, 2020·Chemical Society Reviews·Guangxue FengDan Ding
Jan 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Na-Eun ChoiJiyoun Lee
Aug 20, 2020·Chemical Reviews·Hongjian HeBing Xu
May 25, 2021·Chemical Society Reviews·Hao-Ran JiaFu-Gen Wu
Aug 22, 2018·ACS Applied Materials & Interfaces·Yuhui LiaoDa Xing
Sep 29, 2018·Analytical Chemistry·Hao QiuXiaogang Qu
Aug 10, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Enming DuYe Zhang
Aug 24, 2021·ACS Applied Materials & Interfaces·Tarushyam MukherjeeMayeul Collot
Apr 4, 2020·Analytical Chemistry·Penghui ChengKanyi Pu
Oct 20, 2018·Analytical Chemistry·Chamari S WijesooriyaEmily A Smith
Jul 16, 2020·ACS Applied Materials & Interfaces·Ya-Ping WangCheng-Yong Su
Oct 13, 2020·Analytical Chemistry·Dmytro I DanylchukAndrey S Klymchenko
Jul 14, 2020·ACS Omega·Shuang Liu, Bing Xu
Jan 9, 2021·Journal of the American Chemical Society·Dmytro I DanylchukAndrey S Klymchenko
Jul 12, 2018·Journal of the American Chemical Society·Zhaoqianqi FengBing Xu

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