PMID: 40223Jul 1, 1979

Active transport of calcium in Neurospora plasma membrane vesicles

Proceedings of the National Academy of Sciences of the United States of America
P Stroobant, G A Scarborough

Abstract

Functionally inverted plasma membrane vesicles isolated from the eukaryotic microorganism Neurospora crassa catalyze Mg2+/ATP-dependent Ca2+ uptake. Inhibitors induced efflux studies and isotope-exchange experiments indicate that the Ca2+ is accumulated inside the vesicles against a concentration gradient of about 40-fold, and that the majority of the transported Ca2+ is present essentially in free solution. Comparisons of Mg2+/ATP-driven 45Ca2+ uptake and [14C]SCN-uptake with respect to the Mg2+/ATP concentration dependence, the effects of inhibitors, and the nucleotide and divalent cation specificities indicate that the energy for Ca2+ accumulation is derived from ATP hydrolysis catalyzed by the electrogenic plasma membrane ATPase. Energized Ca2+ uptake is stimulated by the permeant anion SCN- to a degree that varies reciprocally with the ability of this anion to dissipate the membrane potential, and is inhibited by K+ in the presence of nigericin. All of these data point to the conclusion that the active transport of Ca2+ across the Neurospora plasma membrane takes place via a Ca2+/H+ antiporter, which functions to pump Ca2+ out of the intact cell.

References

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Citations

Apr 30, 1977·Archives of Biochemistry and Biophysics·G A Scarborough
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G A Scarborough
Apr 28, 1978·Annals of the New York Academy of Sciences·A N MartonosiA Schibeci
Jun 1, 1979·Analytical Biochemistry·P Stroobant, G A Scarborough
Apr 28, 1978·Annals of the New York Academy of Sciences·L Moore, I Pastan
Feb 1, 1978·Archives of Biochemistry and Biophysics·J W Belliveau, J K Lanyi
Jan 1, 1976·Annual Review of Biochemistry·B C Pressman
Jan 1, 1972·Annual Review of Biophysics and Bioengineering·G Inesi
Jun 15, 1974·European Journal of Biochemistry·E J HarrisI Landaeta
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Gross, D Marmé

Related Concepts

Microorganism
Neurospora crassa
Cations, Divalent
Calcium [EPC]
Antiporter
Neurospora
Calcium
Adenosine Triphosphatases
Uptake
Pandavir

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