Active uptake of tetracycline by membrane vesicles from susceptible Escherichia coli.

Antimicrobial Agents and Chemotherapy
L M McMurryS B Levy

Abstract

A major portion of tetracycline accumulation by susceptible bacterial cells is energy dependent. Inner membrane vesicles prepared from susceptible Escherichia coli cells concentrated tetracycline 2.5 to 5 times above the external concentration when the electron transport substrate D-lactate or reduced phenazine methosulfate was added. This stimulation was reversed by cyanide, 2,4-dinitrophenol, and carbonyl cyanide m-chlorophenyl hydrazone. These vesicles data showed that proton motive force alone could energize tetracycline uptake. The lactate-dependent uptake had a pH optimum of 6.9 and a magnesium optimum of 1 mM and was not saturable up to 400 microM tetracycline. Although the vesicles were not as active as cells in concentrating tetracycline, they were less active to a similar extent in concentrating tetracycline, they were less active to a similar extent in concentrating proline, the transport of which is known to be solely proton motive force dependent. Therefore, we concluded that the active uptake of tetracycline in susceptible cells was largely, if not solely, energized by proton motive force.

References

Aug 4, 1976·Journal of the American Chemical Society·G L Asleson, C W Frank
Feb 1, 1977·Journal of Bacteriology·L W Adler, B P Rosen
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J S HongM A Lieberman
Dec 1, 1976·Journal of Pharmaceutical Sciences·E C Newman, C W Frank
Jan 1, 1976·Journal of Supramolecular Structure·J Weckesser, J A Magnuson
Jun 1, 1972·Bacteriological Reviews·F M Harold
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·E A Berger
Sep 5, 1972·Biochemical and Biophysical Research Communications·A M Reynard, L F Nellis
Jan 1, 1974·Methods in Enzymology·H R Kaback
Dec 6, 1974·Science·H R Kaback
Sep 1, 1973·Antimicrobial Agents and Chemotherapy·D Sompolinsky, J Krausz
Jun 1, 1971·The Biochemical Journal·T J Franklin
Oct 1, 1969·Journal of Pharmaceutical Sciences·J L Colaizzi, P R Klink
Jan 1, 1970·The Biochemical Journal·T J Franklin, B Higginson
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·S Sarkar, R E Thach
Mar 13, 1980·Biochemical and Biophysical Research Communications·P R BallI Chopra
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L McMurryS B Levy
Mar 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·M HIEROWSKI
Jan 1, 1965·The Biochemical Journal·T J FRANKLIN, A GODFREY

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Citations

Apr 1, 1985·Archives of Microbiology·M Argast, C F Beck
Apr 1, 1984·Antimicrobial Agents and Chemotherapy·M C Smith, I Chopra
Aug 1, 1984·Antimicrobial Agents and Chemotherapy·M Argast, C F Beck
Nov 1, 1986·Antimicrobial Agents and Chemotherapy·M C RobertsG E Kenny
May 1, 1987·Antimicrobial Agents and Chemotherapy·J L Burns, A L Smith
Jan 1, 1991·Antimicrobial Agents and Chemotherapy·A YamaguchiT Sawai
Mar 1, 1995·Antimicrobial Agents and Chemotherapy·C PelletierP Bourlioux
Jan 1, 1983·Antimicrobial Agents and Chemotherapy·M C Smith, I Chopra
Mar 6, 2012·Biochimica Et Biophysica Acta·Catalin ChimerelDavid K Summers
Jun 2, 2016·Antimicrobial Agents and Chemotherapy·Chinwe U Chukwudi
Apr 1, 1984·Journal of Bacteriology·G R MunskeJ A Magnuson
Mar 1, 1987·Microbiological Reviews·B R Lyon, R Skurray
Jan 10, 2019·ACS Infectious Diseases·Marcella WidyaDavid A Six

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