Abstract
Epidemiological studies suggest an inverse correlation between dietary calcium (Ca(2+)) and vitamin D intake and the risk of colorectal cancer (CRC). It has been shown in vitro that the active vitamin D metabolite, 1,25-dihydroxyvitamin D3 (1,25-D3) can upregulate expression of the calcium-sensing receptor (CaSR). In the colon, CaSR has been suggested to regulate proliferation of colonocytes. However, during tumorigenesis colonic CaSR expression is downregulated and we hypothesized that the loss of CaSR could influence the anti-tumorigenic effects of Ca(2+) and vitamin D. Our aim was to assess the impact of CaSR expression and function on the anti-neoplastic effects of 1,25-D3 in colon cancer cell lines. We demonstrated that in the healthy colon of mice, high vitamin D diet (2500 IU/kg diet) increased expression of differentiation and apoptosis markers, decreased expression of proliferation markers and significantly upregulated CaSR mRNA expression, compared with low vitamin D diet (100 IU/kg diet). To determine the role of CaSR in this process, we transfected Caco2-15 and HT29 CRC cells with wild type CaSR (CaSR-WT) or a dominant negative CaSR mutant (CaSR-DN) and treated them with 1,25-D3 alone, or in combination with CaSR ac...Continue Reading
References
Apr 1, 1995·Cell Proliferation·R R BurasS R Evans
Aug 9, 1996·The Journal of Biological Chemistry·M BaiE M Brown
Jan 31, 1998·The Journal of Clinical Investigation·M WadaN Nagano
Apr 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y SheininH S Cross
Jan 13, 2001·Gut·M AntiG Gasbarrini
May 31, 2002·The Journal of Biological Chemistry·Lucie Canaff, Geoffrey N Hendy
Dec 5, 2003·Journal of the National Cancer Institute·Maria V GrauDavid Heber
Feb 1, 2005·Molecular and Cellular Probes·Claudia RubieMartin Schilling
May 21, 2005·Carcinogenesis·Enikö KállayHeide S Cross
Dec 15, 2005·Cancer·Peter R HoltKan Yang
Jul 22, 2009·International Journal of Cancer. Journal International Du Cancer·Guangming LiuSubhas Chakrabarty
Nov 19, 2010·Cancer Research·Thomas U AhearnRoberd M Bostick
Nov 19, 2011·The Journal of Biological Chemistry·Osvaldo ReyEnrique Rozengurt
Jan 27, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Veronika FedirkoMazda Jenab
Sep 18, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Doris Maria HummelEnikö Kallay
Oct 26, 2012·Anti-cancer Agents in Medicinal Chemistry·Julia HöbausEnikö Kallay
Dec 27, 2012·Biochimica Et Biophysica Acta·Sarah C BrennanEnikö Kallay
Mar 7, 2013·International Journal of Cancer. Journal International Du Cancer·Julia HöbausEnikö Kallay
Nov 2, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Irfete S FetahuEnikő Kállay
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Apr 3, 2014·International Journal of Cancer. Journal International Du Cancer·Irfete S FetahuEnikő Kállay
May 21, 2014·FEBS Letters·Sam X ChengMansour Mohamadzadeh
Citations
Apr 29, 2016·Journal of Cellular Biochemistry·Elżbieta Gocek, George P Studzinski
Nov 3, 2016·Frontiers in Physiology·Abhishek Aggarwal, Enikö Kállay
Nov 20, 2016·Molecular Carcinogenesis·Laia MasvidalCarmen de Torres
Feb 17, 2017·Future Medicinal Chemistry·Pawin PongkorpsakolChatchai Muanprasat
Mar 28, 2018·International Journal of Molecular Sciences·Andrea Gerbino, Matilde Colella
Aug 16, 2018·The British Journal of Nutrition·Guangmang LiuJing Wang
Dec 18, 2018·PloS One·Yasheen GaoRoberd M Bostick
Jan 1, 2017·Journal of Cancer Prevention & Current Research·Karen L ChenZeynep Madak-Erdogan
Jun 25, 2016·The Cancer Journal·Vicente Morales-OyarvideKimmie Ng
Aug 29, 2020·Cancers·Asunción Fernández-BarralAlberto Muñoz
Apr 24, 2018·BioMed Research International·Fang HeYuanyi Peng
Dec 5, 2018·Digestive Diseases and Sciences·Hemant GoyalGiuseppe Lippi
Oct 3, 2018·World Journal of Gastroenterology : WJG·Luca IamartinoMartin Schepelmann
Feb 6, 2020·Molecular Reproduction and Development·Cong LiuYunpeng Hou
Dec 19, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Akhmed AslamBassem Refaat
Aug 31, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Luca IamartinoEnikö Kallay
Nov 5, 2020·Curēus·Moiz JavedIan H Rutkofsky