Active volume regulation in adhered cells

Proceedings of the National Academy of Sciences of the United States of America
Ram M Adar, Samuel A Safran

Abstract

Recent experiments reveal that the volume of adhered cells is reduced as their basal area is increased. During spreading, the cell volume decreases by several thousand cubic micrometers, corresponding to large pressure changes of the order of megapascals. We show theoretically that the volume regulation of adhered cells is determined by two concurrent conditions: mechanical equilibrium with the extracellular environment and a generalization of Donnan (electrostatic) equilibrium that accounts for active ion transport. Spreading affects the structure and hence activity of ion channels and pumps, and indirectly changes the ionic content in the cell. We predict that more ions are released from the cell with increasing basal area, resulting in the observed volume-area dependence. Our theory is based on a minimal model and describes the experimental findings in terms of measurable, mesoscale quantities. We demonstrate that two independent experiments on adhered cells of different types fall on the same master volume-area curve. Our theory also captures the measured osmotic pressure of adhered cells, which is shown to depend on the number of proteins confined to the cell, their charge, and their volume, as well as the ionic content. T...Continue Reading

References

May 1, 1988·Renal Physiology and Biochemistry·A D Macknight
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Jun 25, 2008·Annual Review of Biophysics·Gary J Doherty, Harvey T McMahon
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
Apr 3, 2009·Nature Reviews. Molecular Cell Biology·David C Gadsby
Jan 5, 2011·Nature·Martin P StewartAnthony A Hyman
Apr 29, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·A Philipse, A Vrij
Jun 2, 2011·Results and Problems in Cell Differentiation·Andrew G Clark, Ewa Paluch
Aug 13, 2013·Biophysical Journal·Hongyuan Jiang, Sean X Sun
May 16, 2015·Science·Miriam B GinzbergMarc Kirschner
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ming GuoDavid A Weitz
Feb 8, 2018·Biophysical Journal·Kenan XieHongyuan Jiang

❮ Previous
Next ❯

Citations

Dec 3, 2020·Nature Communications·Eoin McEvoyVivek B Shenoy
Mar 5, 2021·Current Opinion in Cell Biology·Nishit SrivastavaMatthieu Piel
May 5, 2021·ELife·Gaurav BajpaiSamuel Safran

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.