Active VSG expression sites in Trypanosoma brucei are depleted of nucleosomes.

Eukaryotic Cell
Tara M Stanne, Gloria Rudenko

Abstract

African trypanosomes regulate transcription differently from other eukaryotes. Most of the trypanosome genome is constitutively transcribed by RNA polymerase II (Pol II) as large polycistronic transcription units while the genes encoding the major surface proteins are transcribed by RNA polymerase I (Pol I). In bloodstream form Trypanosoma brucei, the gene encoding the variant surface glycoprotein (VSG) coat is expressed in a monoallelic fashion from one of about 15 VSG bloodstream form expression sites (BESs). Little is known about the chromatin structure of the trypanosome genome, and the chromatin state of active versus silent VSG BESs remains controversial. Here, we determined histone H3 occupancy within the genome of T. brucei, focusing on active versus silent VSG BESs in the bloodstream form. We found that histone H3 was most enriched in the nontranscribed 50-bp and 177-bp repeats and relatively depleted in Pol I, II, and III transcription units, with particular depletion over promoter regions. Using two isogenic T. brucei lines containing marker genes in different VSG BESs, we determined that histone H3 is 11- to 40-fold depleted from active VSG BESs compared with silent VSG BESs. Quantitative PCR analysis of fractionate...Continue Reading

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Citations

May 30, 2012·Expert Reviews in Molecular Medicine·Sam AlsfordMark C Field
Nov 16, 2010·Nucleic Acids Research·Mani Shankar NarayananGloria Rudenko
Jan 31, 2013·Nucleic Acids Research·Mani Shankar Narayanan, Gloria Rudenko
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Oct 26, 2011·Essays in Biochemistry·Gloria Rudenko
Jul 31, 2019·Proceedings of the National Academy of Sciences of the United States of America·James BudzakGloria Rudenko

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African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.