Activin A expression regulates multipotency of mesenchymal progenitor cells.

Stem Cell Research & Therapy
F DjouadRocky S Tuan

Abstract

Bone marrow (BM) stroma currently represents the most common and investigated source of mesenchymal progenitor cells (MPCs); however, comparable adult progenitor or stem cells have also been isolated from a wide variety of tissues. This study aims to assess the functional similarities of MPCs from different tissues and to identify specific factor(s) related to their multipotency. For this purpose, we directly compared MPCs isolated from different adult tissues, including bone marrow, tonsil, muscle, and dental pulp. We first examined and compared proliferation rates, immunomodulatory properties, and multidifferentiation potential of these MPCs in vitro. Next, we specifically evaluated activin A expression profile and activin A:follistatin ratio in MPCs from the four sources. The multidifferentiation potential of the MPCs is correlated with activin A level and/or the activin A:follistatin ratio. Interestingly, by siRNA-mediated activin A knockdown, activin A was shown to be required for the chondrogenic and osteogenic differentiation of MPCs. These findings strongly suggest that activin A has a pivotal differentiation-related role in the early stages of chondrogenesis and osteogenesis while inhibiting adipogenesis of MPCs. This ...Continue Reading

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Citations

Sep 22, 2010·Expert Opinion on Therapeutic Targets·Natasa KovacicAna Marusic
Dec 7, 2010·Journal of Cellular and Molecular Medicine·Wesley M JacksonRocky S Tuan
Jun 29, 2011·Journal of Tissue Engineering and Regenerative Medicine·Juan A MonteroJuan M Hurlé
Jun 4, 2015·Stem Cell Research & Therapy·Andre F SteinertFrank Gohlke
Oct 18, 2016·Frontiers in Physiology·Bipradas RoyHugh M Fentress
Nov 12, 2016·International Journal of Molecular Sciences·Namhee JungSung-Chul Jung
Aug 11, 2017·Muscle & Nerve·Saeyoung ParkSung-Chul Jung
Dec 13, 2018·Physiological Reviews·Enrrico BloiseFernando M Reis
Jul 12, 2019·Scientific Reports·Mohammad-Reza ShokriAmir-Hassan Zarnani
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Zixuan LinHang Lin
Jan 10, 2017·Frontiers in Genetics·Christopher R FellowsAli Mobasheri
Dec 1, 2018·Annals of Dermatology·Jee Woong ChoiKyung Chan Park
Aug 20, 2021·Cellular & Molecular Biology Letters·Michal AradMichal Zalzman

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Methods Mentioned

BETA
density gradient centrifugation
PCR
enzyme-linked immunosorbent assay
ELISA
fluorescence activated cell sorting
fluorescence-activated cell sorting
transfection

Software Mentioned

CellQuest ProTM

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