Abstract
We explored the possible signaling pathway by which activin A induces oocyte maturation. Inhba-overexpressing lentivirus vectors were constructed and transfected into primary granulosa cells in vitro and ovary tissues in vivo in rats. The granulosa cell growth curve was drawn, and antibodies for phospho-Smad2, phospho-Erk5, phospho-Nur77, and stem cell factor (SCF) were prepared for western blot analysis. Protein expression of SCF and C-kit in the rat ovaries was detected by immunohistochemical staining. The rate of granulosa cell proliferation was higher in the Inhba gene overexpression group (INH) than in the control groups (CON group and GFP group) in vitro. Protein expression of SCF and C-kit was higher in the INH group than in the other two groups. phospho-Smad2, phospho-ERK5, P-nur77, and SCF proteins showed positive expression in rat ovarian granulosa cells in each group and were obviously increased in the INH group. Activin A overexpression may promote rat granulosa cell proliferation through Smad2/ERK5/nur77 signaling pathways, and rat granulosa cells overexpressing activin A in vitro showed increased levels of SCF and c-kit proteins.
References
Dec 18, 2012·BMC Systems Biology·Shibin MathewIpsita Banerjee
Jun 19, 2013·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Wang JidongYao Zhenwei
Oct 24, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Larissa M CoutinhoFernando M Reis
Feb 18, 2016·Human Reproduction Update·R Wijayarathna, D M de Kretser
Oct 12, 2016·Experimental Dermatology·Chang H SeoYoung K Sung
May 5, 2017·Journal of Assisted Reproduction and Genetics·Semra KahramanMarkus Montag
Jul 19, 2017·Scientific Reports·Jonas J StaudacherBarbara Jung
Jul 29, 2017·Cancer Letters·Marília I FigueiraSílvia Socorro
Mar 3, 2018·Proceedings of the National Academy of Sciences of the United States of America·Edyta KisielnickaChristian R Eckmann
May 18, 2018·Frontiers in Bioscience (Landmark Edition)·Hidetsugu Kohzaki
May 31, 2018·Current Opinion in Nephrology and Hypertension·Wolfgang Jelkmann
Dec 13, 2018·Physiological Reviews·Enrrico BloiseFernando M Reis
Jan 22, 2019·Cell Stem Cell·Stefano ComazzettoSean J Morrison
Jul 20, 2019·Endocrinology·Kelly L WaltonCraig A Harrison
Aug 17, 2019·Journal of Autoimmunity·Ioannis MorianosGeorgina Xanthou
Aug 23, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Koung Li KimWonhee Suh