PMID: 15225131Jul 1, 2004Paper

Activin and inhibin receptor gene expression in the ewe pituitary throughout the oestrous cycle

The Journal of Endocrinology
A FafioffeJ Dupont

Abstract

In mammals, activin and inhibin are important regulators of FSH secretion. Previous studies have demonstrated that primary ovine pituitary cells express different activin receptor subtypes: activin receptor-like (ALK)2, ALK4, activin type II receptor A (ActRIIA), ActRIIB and Smad proteins in vitro. Here, we have carried out physiological studies to investigate the pattern of mRNA expression of the activin receptor subunits in the ewe pituitary throughout the oestrous cycle. The oestrous cycles of ewes were synchronized with progestagen sponges. The animals were killed 36 h (before the preovulatory surge, n=4), 48 h (during the preovulatory surge, n=4), 72 h (during the second surge of FSH, n=6) and 192 h (during the luteal phase, n=4) after sponge removal. Using Northern blots, we have shown that the levels of ALK2, ALK4 and ActRIIB mRNA were significantly higher before the preovulatory surge and during the secondary surge of FSH as compared with both during the preovulatory surge and the luteal phase, whereas the level of the ActRIIA mRNA was similar throughout the oestrous cycle. Using Western blots we have also demonstrated that the level of phospho-Smad2 did not vary during the reproductive cycle. Inhibin binding protein (I...Continue Reading

Citations

Jun 23, 2009·Gene Expression Patterns : GEP·Christopher MalcuitJesse Mager
Jul 15, 2015·The Journal of Endocrinology·Sjoerd D JoustraGerry T M Wagenaar
Mar 30, 2018·Journal of the Endocrine Society·Daniel J BernardJan M Wit
Mar 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·F J LovicuR U de Iongh
Nov 16, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Melissa LaBonty, Pamela C Yelick

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