Activin receptor mRNA expression by neurons of the avian ciliary ganglion

Journal of Neurobiology
K Kos, J N Coulombe

Abstract

Previous studies have suggested that activin may serve as a neurodifferentiation factor regulating somatostatin expression in neurons of the avian ciliary ganglion (CG). As one aspect of examining the role of activin in CG development, we inquired whether any of the known activin receptors are expressed by developing CG neurons in vivo. In addition, we examined whether activin A mRNA is expressed in the choroid layer and iris of the chicken eye. Oligonucleotide primers were designed for the chicken activin receptor type IIA (cActR-IIA), type IIB (cActR-IIB), and activin A. In reverse-transcription-polymerase chain reaction (rtPCR), an appropriately sized product was amplified from CG cDNA using primers to the cActR-IIA but not the cActR-IIB. Sequencing confirmed the identity of the PCR product as a fragment of the cActR-IIA. It thus appears that mRNA for the type IIA but not the type IIB activin receptor is expressed in the chicken CG. An antisense strand digoxigenin-labeled riboprobe complimentary to a 358-bp portion of the cActR-IIA kinase region hybridized to cells within cryostat sections of embryonic CG. From E6.5-E18, hybridization of this probe appears to be specific for cells with a neuronal morphology. Using rtPCR with...Continue Reading

References

Mar 1, 1976·The Journal of Comparative Neurology·C H Narayanan, Y Narayanan
Mar 16, 1992·Biochemical and Biophysical Research Communications·R LegerskiB de Crombrugghe
Apr 15, 1992·Biochemical and Biophysical Research Communications·C J DonaldsonW W Vale
Aug 1, 1990·Trends in Neurosciences·S C Landis
Jan 1, 1991·Methods in Cell Biology·R M Harland
Jun 21, 1990·Nature·A J van den Eijnden-Van RaaijD Huylebroeck
Apr 26, 1990·Nature·D SchubertW H Fischer
Feb 13, 1987·Biochemical and Biophysical Research Communications·Y EtoH Shibai
Apr 22, 1994·Cell·A Hemmati-Brivanlou, D A Melton
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

May 22, 2003·Progress in Neurobiology·Rae Nishi
Jul 2, 2008·Endocrine Reviews·Buel D Rodgers, Dilip K Garikipati
Oct 13, 2009·Autonomic Neuroscience : Basic & Clinical·K N Cane, C R Anderson
Feb 11, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S KoszinowskiK Krieglstein
Apr 6, 2001·Journal of Neurobiology·K KosJ N Coulombe

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