Activin redux: specification of mesodermal pattern in Xenopus by graded concentrations of endogenous activin B

Development
Olaf PiepenburgJ C Smith

Abstract

Mesoderm formation in the amphibian embryo occurs through an inductive interaction in which cells of the vegetal hemisphere of the embryo act on overlying equatorial cells. The first candidate mesoderm-inducing factor to be identified was activin, a member of the transforming growth factor type beta family, and it is now clear that members of this family are indeed involved in mesoderm and endoderm formation. In particular, Derrière and five nodal-related genes are all considered to be strong candidates for endogenous mesoderm-inducing agents. Here, we show that activin, the function of which in mesoderm induction has hitherto been unclear, also plays a role in mesoderm formation. Inhibition of activin function using antisense morpholino oligonucleotides interferes with mesoderm formation in a concentration-dependent manner and also changes the expression levels of other inducing agents such as Xnr2 and Derrière. This work reinstates activin as a key player in mesodermal patterning. It also emphasises the importance of checking for polymorphisms in the 5' untranslated region of the gene of interest when carrying out antisense morpholino experiments in Xenopus laevis.

References

Jan 1, 1985·Differentiation; Research in Biological Diversity·J C Smith, F M Watt
Jul 27, 1995·Nature·P A Wilson, A Hemmati-Brivanlou
Mar 23, 1995·Nature·M M MatzukA Bradley
Jun 1, 1994·Genes & Development·R A RuppH Weintraub
Oct 6, 1994·Nature·J B GurdonP Lemaire
Aug 1, 1994·Current Opinion in Genetics & Development·R M Harland
Aug 13, 1993·Cell·G H Thomsen, D A Melton
Jun 1, 1993·Developmental Biology·C E DohrmannD A Melton
Jan 1, 1997·Current Biology : CB·S Dyson, J B Gurdon
Apr 15, 1997·Developmental Biology·E M Joseph, D A Melton
Mar 14, 1998·Developmental Biology·C M Jones, J C Smith
Jun 26, 1998·Development Genes and Evolution·L MarchantR Mayor
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·S IemuraN Ueno
Aug 12, 1999·Seminars in Cell & Developmental Biology·L Dale, F C Wardle
Oct 6, 1999·Current Biology : CB·C M JonesR S Beddington
Jan 8, 2000·Developmental Biology·C Papin, J C Smith
Jan 21, 2000·Mechanisms of Development·C Chang, A Hemmati-Brivanlou
Jul 31, 2001·Genesis : the Journal of Genetics and Development·S L NuttE Amaya
Jan 11, 2002·Developmental Biology·Yasuko OnumaMakoto Asashima
Nov 28, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jeremy Green
Nov 28, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mustafa K KhokhaTimothy C Grammer

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Citations

Sep 18, 2013·Development Genes and Evolution·Uma Karadge, Richard P Elinson
Jun 1, 2005·Cytokine & Growth Factor Reviews·Christopher M ButlerGail P Risbridger
Apr 19, 2006·Nature Reviews. Genetics·David Kimelman
Jan 17, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J C SmithP H Williams
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·James C Smith
May 18, 2006·Annual Review of Cell and Developmental Biology·J B Gurdon
May 18, 2007·BMC Developmental Biology·Yasushi Saka, James C Smith
Feb 6, 2009·PLoS Biology·Aditya J SethiLynne M Angerer
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Seth DonougheCassandra G Extavour
Dec 17, 2015·Cellular and Molecular Life Sciences : CMLS·Clemens KieckerEsther Bell
Oct 28, 2015·Developmental Cell·Martin BlumChristof Niehrs
Feb 17, 2006·The EMBO Journal·Adrian E HarringtonMarko Hyvönen
Oct 10, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T KinoshitaJ B Gurdon
May 25, 2015·Seminars in Cell & Developmental Biology·A J Durston
Mar 1, 2006·Seminars in Cell & Developmental Biology·Stéphanie Lebreton, C Michael Jones
Jan 24, 2006·Seminars in Cell & Developmental Biology·Samantha Carruthers, Derek L Stemple
Dec 17, 2005·Seminars in Cell & Developmental Biology·Fiona C Wardle, James C Smith
Oct 6, 2009·Developmental Biology·Oliver NentwichJ C Smith
Nov 9, 2004·Current Biology : CB·P Huw WilliamsJames C Smith
Jul 31, 2007·Cell·Michel Kerszberg, Lewis Wolpert
Aug 2, 2005·Developmental Biology·Laura W GamerVicki Rosen
Mar 11, 2009·Developmental Biology·Christopher E BartonJennifer A Pietenpol
Jul 26, 2008·Developmental Biology·Megumi HashiguchiNoriyoshi Sakai
Jun 11, 2017·Cold Spring Harbor Perspectives in Biology·Joseph ZinskiMary C Mullins
Dec 20, 2007·The Journal of Biological Chemistry·Jonathan A KelberPeter C Gray
Oct 23, 2019·Nature Communications·Matias WagnerRebecca Schüle
Sep 12, 2008·Current Protocols in Stem Cell Biology·Jim SmithRoger Patient
Mar 11, 2006·Development·Janet Heasman
Nov 2, 2014·Development·William T ChiuKen W Y Cho
Apr 12, 2008·Development·Judith S Eisen, James C Smith

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