PMID: 429207Feb 21, 1979Paper

Activities of DNA polymerases and RNA polymerases detected in situ in growing and differentiating cells of root cortex

Histochemistry
M J Olszewska, A K Kononowicz

Abstract

Activities of DNA polymerases and RNA polymerases were studied by autoradiographic methods in growing and differentiating root cortex cells of Zea mays - a species in which endomitosis occurs - and Tulipa kaufmanniana - in which this process does not occur. In Tulipa kaufmanniana, the highest activity of DNA polymerase appears in the nuclei of meristematic zone during the S phase of the cell cycle. In Zea mays, endomitotic replication of DNA occurs in all growth and differentiation zones and the activity of DNA polymerase in the nuclei is similar to that in the meristematic zone. In both species, nuclear RNA synthesis, measured with 3H uridine incorporation, is highest in the meristematic zone and declines steadily with development. Activity of nuclear RNA polymerase is present in all developmental zones in both species and is similar to that in the meristematic zone. 3H uridine incorporation into nucleoli decreases markedly in both species, whereas the activity of nucleolar RNA polymerase remains at a high level in all root segments in Zea mays and decreases slightly in Tulipa kaufmanniana. It is argued that the differences between the incorporation of 3H uridine and that or 3H UMP may be caused by a reduction of the pool of e...Continue Reading

References

Feb 1, 1978·Experimental Cell Research·G P Moore
May 1, 1977·Experimental Cell Research·C Krüger, K H Seifart
Oct 15, 1976·Experimental Cell Research·G MorcilloG Giménez-Martín
Sep 5, 1973·Biochemical and Biophysical Research Communications·V L Dunham, J H Cherry
Feb 1, 1971·European Journal of Biochemistry·H MadreiterC Mittermayer
Sep 1, 1970·Experimental Cell Research·F Ruch, A Rosselet
Jul 16, 1970·Biochimica Et Biophysica Acta·E R Stout, M Q Arens
Jun 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·J VAN'T HOF, A H SPARROW

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