PMID: 12801458Jun 13, 2003Paper

Activities of enzymes in the malate-aspartate shuttle and the isoenzyme pattern of lactate dehydrogenase in plasma and peripheral leukocytes of lactating Holstein cows and riding horses

Research in Veterinary Science
T AraiN Kimura

Abstract

The activities of the enzymes involved in the malate-aspartate shuttle and lactate dehydrogenase (LDH) and the pattern of the isoenzymes of LDH were determined in plasma and peripheral leukocytes of lactating Holstein cows and thoroughbred riding horses as representative herbivorous animals. In the horse plasma, LDH activities were significantly lower and AST activities were significantly higher than those in the cow plasma. The specific activities of cytosolic malate dehydrogenase (MDH), LDH and AST in the horse leukocytes were higher than those in the cows. The cytosolic ratio of MDH/LDH activity (ML ratio) in the horse leukocytes was significantly lower than that in the cow leukocytes owing to significantly higher activities of LDH. The ML ratio was considered to reflect the difference in energy metabolism in leukocytes between cows and horses. The plasma LDH isoenzyme patterns of cow and horse showed the characteristic as herbivorous animals with dominance of LDH-1, -2 and -3. The LDH isoenzyme patterns with dominance of LDH-3 and -4 in the horse leukocytes were remarkably different from those in the cow leukocytes. There were significant differences in activities of malate-aspartate shuttle enzymes, ML ratio and LDH isoenz...Continue Reading

References

Jan 1, 1989·Veterinary Research Communications·T AraiY Oki
Sep 14, 1987·Biochimica Et Biophysica Acta·R Hernández-MuñozV Chagoya de Sánchez
Feb 1, 1982·Archives of Biochemistry and Biophysics·M J MacDonald
Apr 1, 1956·Proceedings of the Society for Experimental Biology and Medicine·A SIEGEL, R J BING

Citations

Mar 6, 2009·In Vitro Cellular & Developmental Biology. Animal·Lin-feng LiYuehui Ma
Aug 31, 2013·In Vitro Cellular & Developmental Biology. Animal·Jun LuoSheng-sheng Lu
Jun 27, 2008·Tissue & Cell·Chang-Qing LiuXuexi Tang
Jul 7, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Chang-Qing LiuYue-Hui Ma

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