PMID: 9551600Apr 29, 1998Paper

Activities of SV40 T antigen necessary for the induction of tetraploid DNA content in permissive CV-1 cells

Cytometry
M B Perry, J M Lehman

Abstract

To determine the role of SV40 T antigen in stimulating multiple rounds of DNA synthesis in permissive cells, CV-1 cells were transfected with plasmids expressing mutant or wt T antigen in the presence or absence of the SV40 origin of replication. Induction of cells with > G2 DNA content (tetraploid DNA content) and levels of T antigen protein were detected and analyzed by flow cytometry. The mutant T antigen proteins demonstrated the expected phenotypes as determined by immunoprecipitation. Elevated levels of T antigen protein were detected in each transfection, but full-length T antigen alone was responsible for the tetraploid DNA content. The studies show that full-length T antigen with point mutations to reduce binding to the cellular proteins p53 and/or Rb were capable of inducing > G2 DNA content though the induction by these mutants was greatly enhanced by the presence of the SV40 origin of replication. Truncated T antigen (aa 1-259) could induce cells with tetraploid DNA content only in the presence of the SV40 origin of replication and the absence of Rb binding. These studies suggest that multiple functions of T antigen are involved in the stimulation of the second round of cellular DNA synthesis.

Citations

Jun 4, 2004·Genes & Development·Xiaohua WuDavid Livingston
Apr 27, 2010·PLoS Computational Biology·M Rowan BrownRachel J Errington
Feb 4, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·M Rowan BrownRachel J Errington
Aug 10, 1999·Experimental Cell Research·B WhalenJ M Lehman
Aug 16, 2018·PloS One·Melvys ValledorPaul C Schiller
Jan 5, 2011·Protein & Cell·Chonghua Li, Jianping Jin

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