Activities of the matrix metalloproteinase stromelysin-2 (MMP-10) in matrix degradation and keratinocyte organization in wounded skin

Molecular Biology of the Cell
Monika KrampertS Werner

Abstract

The matrix metalloproteinase stromelysin-2 is expressed in keratinocytes of the epithelial tongue of skin wounds, suggesting a role in keratinocyte migration. Here, we show that stromelysin-2 enhances migration of cultured keratinocytes. To gain insight into the in vivo activities of stromelysin-2 in epithelial repair, we generated transgenic mice expressing a constitutively active stromelysin-2 mutant in keratinocytes. These animals had no alterations in skin architecture, and the healing rate of skin wounds was normal. Histologically, however, we found abnormalities in the organization of the wound epithelium. Keratinocytes at the migrating epidermal tip were scattered in most sections of mice with high expression level, and there was a reduced deposition of new matrix. In particular, the staining pattern of laminin-5 at the wound site was altered. This may be due to proteolytic processing of laminin-5 by stromelysin-2, because degradation of laminin-5 by this enzyme was observed in vitro. The inappropriate matrix contact of keratinocytes was accompanied by aberrant localization of beta1-integrins and phosphorylated focal adhesion kinase, as well as by increased apoptosis of wound keratinocytes. These results suggest that a t...Continue Reading

References

Mar 1, 1978·European Journal of Biochemistry·R TimplH Wiedemann
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R VassarE Fuchs
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Jul 1, 1994·The Journal of Clinical Investigation·U K Saarialho-KereH G Welgus
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K MiyazakiM Umeda
Jul 1, 1993·The Journal of Investigative Dermatology·A B WysockiF Grinnell
Jul 1, 1996·The British Journal of Dermatology·M VaalamoU K Saarialho-Kere
Aug 14, 1999·Science·F G Giancotti, E Ruoslahti
Jan 13, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·W C Parks
Feb 9, 2000·The Journal of Cell Biology·N KoshikawaV Quaranta
May 3, 2000·The Journal of Investigative Dermatology·R CaldelariE Müller
Aug 6, 2000·The Journal of Biological Chemistry·B P NguyenW G Carter
Sep 9, 2000·Current Opinion in Cell Biology·B P NguyenW G Carter
Nov 9, 2000·The Journal of Investigative Dermatology·O RechardtU Saarialho-Kere
May 18, 2001·Oral Diseases·B Birkedal-HansenW G Stetler-Stevenson
Sep 7, 2001·Current Opinion in Cell Biology·L J McCawley, L M Matrisian
Nov 1, 2001·Annual Review of Cell and Developmental Biology·M D Sternlicht, Z Werb
Apr 11, 2002·Nature Cell Biology·Cindy K Miranti, Joan S Brugge
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
Sep 18, 2002·Journal of Cell Science·Dwayne G Stupack, David A Cheresh
Sep 25, 2002·Cancer Detection and Prevention·Robin MathewRanju Ralhan
Apr 10, 2003·Biochemical and Biophysical Research Communications·Emma PiriläPäivi Maisi
Jun 28, 2003·FEBS Letters·Michael P Scheid, James R Woodgett
Mar 5, 2004·Journal of Cell Science·Diane E Frank, William G Carter
May 27, 2004·The American Journal of Pathology·Kaisa TasanenMonique Aumailley
Jun 24, 2004·Journal of Cellular Biochemistry·Takashi OgawaKaoru Miyazaki
Jun 24, 2004·Experimental Cell Research·Yoshinobu Kariya, Kaoru Miyazaki

❮ Previous
Next ❯

Citations

Apr 25, 2012·Cell and Tissue Research·Vera L MartinsEdel A O'Toole
Jul 20, 2010·Amino Acids·T Klein, R Bischoff
Aug 21, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Rugmani Padmanabhan IyerMerry L Lindsey
Sep 28, 2005·Investigative Ophthalmology & Visual Science·Mehrnoosh SaghizadehAlexander V Ljubimov
May 22, 2013·PloS One·Megan Y MurrayJelena Gavrilovic
Jul 18, 2014·Advances in Wound Care·Irena PastarMarjana Tomic-Canic
Jan 1, 2009·Journal of Oncology·Jennifer FreytagPaul J Higgins
Jan 18, 2008·Journal of Pharmaceutical Sciences·Jose L Mauriz, Javier González-Gallego
Jan 24, 2016·The Journal of Investigative Dermatology·Jungtae NaBong-Gun Ju
Oct 31, 2006·The Journal of Investigative Dermatology. Symposium Proceedings·George E Davis, W Brian Saunders
Nov 22, 2011·Molecular Aspects of Medicine·Diego SbardellaMassimo Coletta
Oct 16, 2009·Brain Research Bulletin·Mehrnoosh SaghizadehAlexander V Ljubimov
Jun 29, 2005·The Journal of Investigative Dermatology·Jens M BaronFrank K Jugert
Dec 16, 2005·The Journal of Investigative Dermatology·Andrea K SmileyDorothy M Supp
Mar 8, 2008·The Journal of Investigative Dermatology·Chieh-Fang ChengWei Li
Sep 12, 2015·The Journal of Investigative Dermatology·Liisa M Nissinen, Veli-Matti Kähäri
May 1, 2015·The Journal of Investigative Dermatology·Maryam G RohaniWilliam C Parks
Mar 27, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Oihane García-IrigoyenMatias A Avila
Sep 15, 2007·The British Journal of Dermatology·T M JärvinenU Saarialho-Kere
Jan 25, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Emma PiriläTimo Sorsa
Nov 16, 2007·Journal of Cutaneous Pathology·Ville BisterUlpu Saarialho-Kere
Jan 25, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Alan B G LansdownMagnus S Agren
Apr 20, 2006·Birth Defects Research. Part C, Embryo Today : Reviews·Vincent Lemaître, Jeanine D'Armiento
Dec 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Helen H N YanC Yan Cheng
Sep 23, 2008·Experimental Dermatology·Sara A IllmanJorma Keski-Oja
Oct 15, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Oihane Garcia-IrigoyenMatías A Ávila
Jul 16, 2009·Experimental Dermatology·Sonja BoydUlpu Saarialho-Kere
Oct 3, 2009·Journal of Cellular Biochemistry·Peter Chen, William C Parks
Sep 6, 2006·Experimental Cell Research·Togo Ikuta, Kaname Kawajiri
Sep 30, 2015·Respiratory Research·Akihiko SokaiMichiaki Mishima

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