Activity and diversity of sulfate-reducing bacteria in a petroleum hydrocarbon-contaminated aquifer.

Applied and Environmental Microbiology
J KleikemperJosef Zeyer

Abstract

Microbial sulfate reduction is an important metabolic activity in petroleum hydrocarbon (PHC)-contaminated aquifers. We quantified carbon source-enhanced microbial SO(4)(2-) reduction in a PHC-contaminated aquifer by using single-well push-pull tests and related the consumption of sulfate and added carbon sources to the presence of certain genera of sulfate-reducing bacteria (SRB). We also used molecular methods to assess suspended SRB diversity. In four consecutive tests, we injected anoxic test solutions (1,000 liters) containing bromide as a conservative tracer, sulfate, and either propionate, butyrate, lactate, or acetate as reactants into an existing monitoring well. After an initial incubation period, 1,000 liters of test solution-groundwater mixture was extracted from the same well. Average total test duration was 71 h. We measured concentrations of bromide, sulfate, and carbon sources in native groundwater as well as in injection and extraction phase samples and characterized the SRB population by using fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE). Enhanced sulfate reduction concomitant with carbon source degradation was observed in all tests. Computed first-order rate coe...Continue Reading

References

Feb 1, 1992·Applied and Environmental Microbiology·R I AmannD A Stahl
Mar 1, 1992·Applied and Environmental Microbiology·E A EdwardsD Grbić-Galić
Nov 1, 1996·Applied and Environmental Microbiology·A FelskeH Backhaus
Oct 6, 1998·Applied and Environmental Microbiology·C M Santegoeds de Beer D
Jan 29, 2000·Biotechnology and Bioengineering·R Kleerebezem, A J Stams
Jul 27, 2001·Applied and Environmental Microbiology·W V SiglerR F Turco
Jul 1, 1981·Applied and Environmental Microbiology·J SørensenB B Jørgensen

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Citations

Jul 10, 2003·Current Opinion in Biotechnology·Barth F Smets, P H Pritchard
Oct 24, 2008·Environmental Technology·S M Tiquia
Jul 19, 2011·Applied and Environmental Microbiology·M MilettoD R Lovley
Sep 25, 2012·Applied and Environmental Microbiology·Min Sub SimTanja Bosak
Jul 5, 2011·Applied and Environmental Microbiology·Helmut BürgmannKai M Udert
Oct 9, 2003·Applied and Environmental Microbiology·J J RichD D Myrold
Jan 11, 2005·Applied and Environmental Microbiology·Jutta KleikemperJosef Zeyer
Sep 10, 2005·Applied and Environmental Microbiology·Christopher G StruchtemeyerMichael J McInerney
Aug 10, 2005·Canadian Journal of Microbiology·Dan SpiegelmanCharles W Greer
Sep 22, 2009·Canadian Journal of Microbiology·Xiu-Juan WuHong Yang
Jun 14, 2013·PloS One·Magalie StauffertRobert Duran
Sep 14, 2006·International Journal of Environmental Research and Public Health·Enid M Rodríguez-MartínezArturo A Massol-Deyá
Dec 17, 2008·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Mathava KumarJih-Gaw Lin
May 23, 2012·3 Biotech·Chioma Blaise ChikereBlaise Ositadinma Chikere
Sep 27, 2014·Environmental Science and Pollution Research International·Magalie StauffertRobert Duran
Oct 20, 2005·Environmental Microbiology·Helmut BürgmannJosef Zeyer
May 27, 2004·Ground Water·Young KimLewis Semprini
Mar 25, 2006·Ground Water·Martin H Schroth, Jonathan D Istok
Jan 16, 2008·Ground Water·Johanna V Weiss, Isabelle M Cozzarelli
Mar 1, 2009·Microbial Biotechnology·Lisa M GiegJoseph M Suflita
Jan 6, 2011·Ground Water·Tara A KneeshawErik W Smith
Sep 17, 2013·Environmental Microbiology·Maryeimy Varon-LopezFernando Dini Andreote
Dec 22, 2004·Journal of Contaminant Hydrology·Kimberly J HagemanLewis Semprini
Sep 1, 2015·Journal of Hazardous Materials·Bidus Kanti DasJayanta Bhattacharya
May 9, 2006·Journal of Contaminant Hydrology·Martha A SchollScott C Christenson
Dec 13, 2005·Journal of Microbiological Methods·Joke GeetsKarolien Vanbroekhoven
Jun 18, 2005·Current Opinion in Biotechnology·Kate M Scow, Kristin A Hicks
Aug 12, 2014·Microbial Ecology·Raeid M M AbedSamiha Al-Kharusi
Sep 23, 2008·FEMS Microbiology Ecology·Sandrine PaisséRobert Duran
May 12, 2009·Advances in Applied Microbiology·Larry L Barton, Guy D Fauque

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